C-terminal Sortagging-[Cys(AF488)]

GGG-[C(AF488)]-amide

  • Description

  • Application Data

Description

Acts as a (oligo)glycine nucleophile in the final steps of a sortagging reaction, which results in the fluorescent dye being attached to the C-terminus of target peptide

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Application Data

Catalogue number crb1110801
Molecular Weight 989.2
Sequence (one letter code)

GGG-[C(AF488)]-amide

Sequence (three letter code)

H-Gly-Gly-Gly-[Cys(AF488)]-NH2

Aliase sortase tagging peptide
Modifications Where AF488 is Alexa Fluor® 488
Purity >95%
References

Strijbis et al (2012) Protein Ligation in Living Cells Using Sortase. Traffic 13(6) 780 PMID: 22348280

Sinisi et al (2012) Development of an Influenza virus Protein Array Using Sortagging Technology. Bioconjug. Chem. 23(6) 1119 PMID: 22594688

Ton-That et al (2000) Anchoring of Surface Proteins to the Cell Wall ofStaphylococcus aureus. J. Biol. Chem. 275(13) 9876 PMID: 10734144

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

This C-terminal Sortagging peptide acts as a (oligo)glycine nucleophile in the final steps of a sortagging protein labelling reaction. This reaction results in the [Cys(AF488)]- fluorescent moiety being attached to the C-terminus of the target protein or peptide.

A substrate peptide containing the LPXTG motif is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA, serves as a nucleophile to cleave the peptide bond between threonine and glycine of the substrate peptide. Cleavage results in the formation of a thioacyl intermediate between the substrate peptide and SrtA. This intermediate is then resolved by the N-terminus of this (oligo)glycine nucleophile peptide, resulting in the creation of a new peptide bond that links the substrate peptide to this peptide and its fluorescent dye.  This method of protein labelling is known as sortagging.

This peptide contains the AF488 fluorescent dye; AF488 is a bright green dye with excitation at 488 nm, it is water soluble and stable from pH 4 to pH 10

C-terminal Sortagging-[Cys(AF488)]

Cat No.Pack SizePriceQty.
0.1mg£190.00
0.5mg£220.00
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