Biotin-LPETGG N-terminal Sortagging Biotin-LPETGG-amide

  • Description

  • Application Data

Description

Substrate peptide for sortagging reaction; for covalently attaching biotin to the N-terminus of the target peptide

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Application Data

Catalogue number crb1000655
Molecular Weight 797.4
Sequence (one letter code) Biotin-LPETGG-amide
Sequence (three letter code) Biotin-Leu-Pro-Glu-Thr-Gly-Gly-NH2
Purity >95%
Storage -20°C
References

Strijbis et al (2012) Protein Ligation in Living Cells Using Sortase. Traffic 13(6) 780 PMID: 22348280

Sinisi et al (2012) Development of an Influenza virus Protein Array Using Sortagging Technology. Bioconjug. Chem. 23(6) 1119 PMID: 22594688

Ton-That et al (2000) Anchoring of Surface Proteins to the Cell Wall ofStaphylococcus aureus. J. Biol. Chem. 275(13) 9876 PMID: 10734144

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

This peptide is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA serves as a nucleophile to cleave the peptide bond between threonine and glycine. Cleavage results in the formation of a thioacyl intermediate between the peptide and SrtA. This intermediate is then resolved by the N-terminus of an (oligo)glycine nucleophile, resulting in the creation of a new peptide bond that links the peptide and its biotin tag to the incoming nucleophile.  This method of protein labelling is known as sortagging.

This peptide contains an N-terminal biotin tag for detection and purification

Biotin-LPETGG N-terminal Sortagging

Cat No.Pack SizePriceQty.
0.5mg£110.00
1mg£140.00
5mg£400.00
10mg£800.00
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