Biotin-Jak2 substrate Biotin-GGEEEEYFELVKK-acid
Phospho-substrate for JAK2/3 for use in kinase assays, contains N-terminal biotin
Catalogue number crb1000246 Molecular Weight 1782.96 Sequence (one letter code) Biotin-GGEEEEYFELVKK-acid Sequence (three letter code) Biotin-Gly-Gly-Glu-Glu-Glu-Glu-Tyr-Phe-Glu-Leu-Val-Lys-Lys-OH Molecular Weight 1782.96 Purity >95% References
Jay et al (2014) JAK2 Tyrosine Kinase Phosphorylates and Is Negatively Regulated by Centrosomal Protein Ninein. Mol. Cell. Biol. 35(1) 111 PMID: 25332239
Liu et al (2017) Cbl-mediated K63-linked ubiquitination of JAK2 enhances JAK2 phosphorylation and signal transduction. Sci. Rep. 7(1) PMID: 28676638
Mishra and Kumar (2014) Adapter Protein Shc Regulates Janus Kinase 3 Phosphorylation. J. Biol. Chem. 289(23) 15951 PMID: 24795043
This peptide is phosphorylated by Janus kinase 2 and 3 (JAK2 and JAK3) and is an ideal substrate for use in kinase assays. The JAK family of kinases is essential for the signalling of a host of immune modulators in tumour, stromal, and immune cells where they are highly expressed. JAK family proteins mediate the signalling of the interferon (IFN), IL-6, and IL-2 families of cytokines.
JAK kinases are associated with cytokine receptors. Cytokine binding to these receptors results in activation of JAK kinases and receptor phosphorylation. Phosphorylated cytokine receptors recruit STAT proteins, which are then phosphorylated by the activated JAK kinases. Phosphorylated STAT proteins form homo- and hetero-dimers that translocate into the nucleus and function as transcription factors.
This peptide contains an N-terminal biotin tag for easy detection and purification.