[Atto655]-LifeAct (Abp140 1-17)
[Atto655]-LifeAct (Abp140 1-17) contains the fluorophore Atto655 and the 17 amino acid peptide Lifeact, whose high affinity for actin filaments allows it to be used as a cytoskeletal marker.
Catalogue number crb1101303 Molecular Weight 2432.2 Sequence (one letter code)
Sequence (three letter code)
Purity >95% Storage -20°C References
Riedl et al (2010) Lifeact: a versatile marker to visualize F-actin. Nat. Methods 5(7) 605 PMID: 18536722
T. Wang et al (2014) The Covalent Trimethoprim Chemical Tag Facilitates Single Molecule Imaging with Organic Fluorophores. Biophys. J. 106 (1) 272 PMID: 24411259
Montes-Rodriguez and Kost. (2017) Direct Comparison of the Performance of Commonly Employed In Vivo F-actin Markers (Lifeact-YFP, YFP-mTn and YFP-FABD2) in Tobacco Pollen Tubes. Front. Plant Sci. 8 1349 PMID: 28824684
Manufactured in: United Kingdom
[Atto655]-LifeAct (Abp140 1-17) contains the 17 amino acid peptide Lifeact derived from amino acids 1-17 of the Saccharomyces cerevisiae actin binding protein, Abp140. These first 17 amino acids of Abp140 are crucial in allowing Lifeact to localise to actin filaments (F-actin) and therefore it can be used as a cytoskeletal marker. One application of lifeact is in the study of plant development and pathogen defence as filamentous actin within the plant’s actin cytoskeleton is important in key processes such as cell division, membrane trafficking and stomatal movements. The addition of the oxazine fluorophore Atto655 which has single molecule (SM) imaging properties allows the location of the LifeAct (Abp140 1-17) to be detected.