Antennapedia peptide amide RQIKIWFQNRRMKWKK-amide
Description
Application Data
Description
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A C-terminal amide cell-penetrating peptide (CPP), derived from the homeodomain of Antennapedia (Drosophila), also known as penetratin.
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1000177 Molecular Weight 2245.75 Sequence (one letter code) RQIKIWFQNRRMKWKK-amide Sequence (three letter code) H-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-NH2
Aliase Penetratin Purity >95% References Derossi et al., (1998). Trojan peptides: the penetratin system for intracellular delivery. Trends Cell Biol., 8(2): 84. DOI: https://doi.org/10.1016/S0962-8924(98)80017-2.
Pietersz et al., (2001). Apostolopoulos V. A 16-mer peptide (RQIKIWFQNRRMKWKK) from antennapedia preferentially targets the Class I pathway. Vaccine., 19(11): 1397. PMID: 11163662.
Manufactured in: United Kingdom Identification of cell penetrating conjugates has aided numerous areas of scientific development. The Drosophila transcription factor Antennapedia contains a homeodomain that can be internalised by cells to the cytoplasm and to the nucleus in a receptor-independent mechanism. The key residues for internalisation have been sequenced (RQIKIWFQNRRMKWKK), named penetratin, and used in several studies to aid entry of fusion proteins into cells.
The full 60 amino acid homeodomain was fused to a T cell epitope of the influenza nucleoprotein and successfully internalised into T cells for presentation. The fragment known as penetratin was fused to a ligand for Grb-2 resulting in inhibition of downstream Grb-2 signalling events. Penetratin has also been used in vivo to prime cytotoxic T lymphocytes by conjugating short antigenic peptides to the CPP. Penetratin is provided here as a C-terminal amide but is also available in acid form.