Fluorogenic substrate for trypsin-like activity. Contains a Rhodamine 110 molecule which will fluoresce upon peptide hydrolysis
Catalogue number crb1100532 Molecular Weight 894.5 Sequence (one letter code) Ac-RLR-[Rh110]-[D-Pro] Sequence (three letter code)
Modifications Where Rh110 is Rhodamine 110 Purity >95% References
Cundiff et al., (2019) Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability. Sci Rep. 9: 14506. PMID: 31601863
Lacy et al., (2018). Identification of an Arg-Leu-Arg tripeptide that contributes to the binding interface between the cytokine MIF and the chemokine receptor CXCR4. Sci Rep. 8: 5171. PMID: 29581527
Manufactured in: United Kingdom
Fluorogenic substrate peptide to assay trypsin-like activity. In its intact state this peptide is non-fluorescent, however when the Rhodamine fluorophore is released upon hydrolysation, fluorescence can be detected. This peptide is therefore a useful tool for analysing trypsin-like enzyme activity.
The presence of the D-proline residue on the C terminal of the rhodamine molecule ensures one directional rhodamine cleavage which simplifies fluorescence studies. Rhodamine 110 is a laser grade fluorescent dye
with excitation maxima at 496 nm and emission maxima at 522 nm.