Ac-GPLD-[Rh110]-[D-Pro]
Ac-GPLD-[Rh110]-[D-Pro]
Description
Application Data
Description
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Fluorogenic substrate for assaying proteasome activity. Contains a Rhodamine 110 molecule which will fluoresce upon peptide hydrolysis
Application Data
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Catalogue number crb1101107 Molecular Weight 851.3 Sequence (one letter code) Ac-GPLD-[Rh110]-[D-Pro]
Sequence (three letter code) Ac-GPLD-[Rh110]-[D-Pro], Where Rh110 is Rhodamine 110
Purity >95% References Banerjee et al., (2019). Inhibition of dual-specificity tyrosine phosphorylation-regulated kinase 2 perturbs 26S proteasome-addicted neoplastic progression. PNAS USA. 116(49): 24881. PMID: 31754034
Manufactured in: United Kingdom Fluorogenic substrate peptide to assay the caspase-like peptidic activity of the 20S proteasome. In its intact state this peptide is non-fluorescent, however when the Rhodamine fluorophore is released upon hydrolysation, fluorescence can be detected. This peptide is therefore a useful tool for analysing enzyme activity.
The presence of the D-proline residue on the C terminal of the rhodamine molecule ensures one directional rhodamine cleavage which simplifies fluorescence studies. Rhodamine 110 is a laser grade fluorescent dye with excitation maxima at 496 nm and emission maxima at 522 nm