Ac-Arg-Gly-Lys(Ac)-AMC 1mg

Ac-RG-[K(Ac)]-AMC

£110.00
  • Description

  • Application Data

Description

Substrate peptide for histone deacetylase (HDAC) enzymes. This peptide contains a C-terminal 7-amino-4-methylcoumarin (AMC) fluorescent tag, which is quenched when linked to the peptide via the amide bond and fluoresces upon cleavage.

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Application Data

Catalogue number crb1101605-1-1
Molecular Weight 600.3
Sequence (one letter code)

Ac-RG-[K(Ac)]-AMC

Sequence (three letter code)

Acetyl-Arg-Gly-Lys(Acetyl)-[AMC]

Modifications Where AMC is 7-amino-4-methylcoumarin
Purity >95%
Storage -20°C
Manufactured in: United Kingdom
Material Safety Data Sheet (MSDS)

Substrate peptide for histone deacetylase (HDAC) enzymes for use in assaying HDAC activity.

Histone deacetylases (HDACs) are a family of enzymes which are highly evolutionary conserved across all eukaryotes. HDACs modify histones by removing acetyl groups from the tail regions. Histone deacetylation is generally associated with reduced gene expression due to a more compact chromatin state less accessibility for transcription factors (TFs). HDACs are essential for many physiological processes including development and cellular homeostasis. They also play an important role in disease states, including neurodegenerative disorders, genetic diseases and cancers.

This peptide is the fluorogenic substrate for assaying histone deacetylase (HDAC) activity in a two-step enzymatic reaction. The assay consists of the initial lysine deacetylation by HDAC followed by the release of the fluorescent group by trypsin. Fluorescence can be detected upon fluorophore release.

Ac-Arg-Gly-Lys(Ac)-AMC 1mg

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