Fluorescein N-terminally labelled Transportan 10, a chimeric cell-penetrating peptide sequence obtained from Galanin and Mastoparan.
Catalogue number crb1100282 Molecular Weight 2540.05 Sequence (one letter code) [5-FAM]-AGYLLGKINLKALAALAKKIL-amide Sequence (three letter code) [5-FAM]-Ala-Gly-Tyr-Leu-Leu-Gly-Lys-Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2 Molecular Weight 2540.05 Purity >95% References
Yandek et al (2007) Mechanism of the Cell-Penetrating Peptide Transportan 10 Permeation of Lipid Bilayers. Biophys. J. 92(7) 2434 PMID: 17218466
TP10 is an amphipathic cell-penetrating peptide (CPPs) known as transportan 10. Its formation involves the use of a lysine residue to form a chimeric linkage between a mastoparan 21-residue peptide, a wasp venon 14-residue peptide and 6-residues derived from the neuropeptide galanin. Structurally TP10 contains only positively charged amino acids along with 4 lysines and an N-terminus. Therefore it will produce a +5 charge under conditions of a neutral pH. It has been found that TP10 may aid molecules in penetrating through the cell membrane barrier through directly interacting with the lipid bilayer. During these interactions with the membrane TP10 will form an amphipathic α-helix. Consequently TP10 can be used in transduction methods.
It contains 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag.