[5-FAM]-TAT (47-57) amide [5-FAM]-YGRKKRRQRRR-amide
Description
Application Data
Description
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[5-FAM]-TAT (47-57) is a cell penetrating cationic peptide derived from the N-terminus of the Tat protein found in the human immunodeficiency virus (HIV). It contains 5-carboxyfluorescein (5-FAM), a widely used green fluorescent tag.
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1100169 Molecular Weight 1917.14 Sequence (one letter code) [5-FAM]-YGRKKRRQRRR-amide Sequence (three letter code) [5-FAM]-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-NH2
Purity >95% References Amani et al (2014) Designing and analyzing the structure of Tat-BoNT/A(1-448) fusion protein: An in silico approach. Mol. Bio. Res. Comm. 3(2) 115 PMID: 30805378
Okuda-Shinagawa et al (2017) Fluorescent and Photosensitizing Conjugates of Cell-Penetrating Peptide TAT(47-57): Design, Microwave-Assisted Synthesis at 60 °C, and Properties. ACS Omega 2(11) 8156 PMID: 30023576
Manufactured in: United Kingdom [5-FAM]-TAT (47-57) is a cell penetrating cationic peptide derived from the N-terminus of the Tat protein, which is a trans-activator of the transcription protein present in the human immunodeficiency virus (HIV). Specifically TAT (47-57) is located within the arginine-rich basic domain 48-60 of the TAT peptide which as a whole has three domains which function to aid HIV through transactivation, DNA binding and nuclear transport. As a cell penetrating peptide (CPP) TAT aids in the cellular uptake of molecules and hence serves a valuable purpose in transduction methods. This property has been demonstrated through its ability of allowing toxins such as the neurotoxin Botulinum neurotoxin Type A, produced by the Clostridium botulinum type A bacteria to penetrate the skin barrier non-invasively. Additionally TAT (47-57) can be used to deliver proteins, fluorophores, chelators and DNA to target cells.
It contains 5-carboxyfluorescein (5-FAM), a widely used green fluorescent tag.