[5-FAM]-PR9 [5-FAM]- FFLIPKGRRRRRRRRR-amide
Pas (Penetration accelerating sequence) Nona arginine cell-penetrating peptide N-terminally labelled with fluorescein.
Catalogue number crb1100285 Molecular Weight 2584.01 Sequence (one letter code) [5-FAM]- FFLIPKGRRRRRRRRR-amide Sequence (three letter code) [5-FAM]-Phe-Phe-Leu-Ile-Pro-Lys-Gly-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH2 Molecular Weight 2584.01 Purity >95% References
Borrelli et al (2018) Cell Penetrating Peptides as Molecular Carriers for Anti-Cancer Agents. Molecules 23 295 doi:10.3390/molecules23020295
Liu et al (2013) Endocytic Trafficking of Nanoparticles Delivered by Cell-penetrating Peptides Comprised of Nona-arginine and a Penetration Accelerating Sequence. PLoS One 8(6) e67100 PMID: 23840594
CPPs can transport molecules such as nucleic acids, proteins and imaging agents into cells of inter-est. PR9, Pas non-arginine, is an arginine rich CPP. It is composed of the nona-arginine: R9 and Pas which is a peptide penetrating accelerating sequence and it functions to export molecules out of endocytic vesicles. During a study in which PR9 was in complex with a Quantum dot probe (QD) it was evident that the PR9/QD complex was transported into the cell through endocytosis where it co-localises with actins, lysosomes, early endosomes and the nucleus. Due to the non-toxicity of the PR9/QD complex it can be used as a safe vector for biomedical purposes.
It contains 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag.