Alpha helical amphipathic model peptide (MAP) labelled at the N-terminus with fluorescein
Catalogue number crb1100286 Molecular Weight 2234.76 Sequence (one letter code) [5-FAM]-KLALKLALKALKAALKLA-amide Sequence (three letter code) [5-FAM]-Lys-Leu-Ala-Leu-Lys-Leu-Ala-Leu-Lys-Ala-Leu-Lys-Ala-Ala-Leu-Lys-Leu-Ala-NH2 Molecular Weight 2234.76 Purity >95% References
Mant et al., (2019). De Novo Designed Amphipathic α-Helical Antimicrobial Peptides Incorporating Dab and Dap Residues on the Polar Face To Treat the Gram-Negative Pathogen, Acinetobacter baumannii. J Med Chem. 62(7): 3354. PMID: 30848594
Kerth et al., (2004). Infrared Reflection Absorption Spectroscopy of Amphipathic Model Peptides at the Air/Water Interface. Biophys J. 86(6): 3750. PMID: 15189871
Amphipathic α-helical cationic antimicrobial peptides (AMPs) are rapidly bactericidal and have broad spectrum activity. As AMPs have non-specific modes of action involving membrane disruption, bacteria are less likely to develop resistance to them (unlike traditional antibiotics).
KLAL is a model compound to form amphipathic helices that are able to bind to membranes and increase the membrane permeability. KLAL model peptides may also form a β-structure under appropriate conditions.
Peptide is labelled with an N-terminal 5-carboxyfluorescein (5-FAM), a widely used green fluorescent tag.