[5-FAM]/[Lys(Dnp)]-SARS-CoV-2 S1/S2 [5-FAM]-TNSPRRARSVA-[K(Dnp)]-amide
Description
Application Data
Description
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FRET substrate peptide derived from SARS-CoV-2 spike (S) S1/S2 site
Application Data
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Material Safety Data Sheet (MSDS)Catalogue number crb1101580 Molecular Weight 1864.8 Sequence (one letter code) [5-FAM]-TNSPRRARSVA-[K(Dnp)]-amide Sequence (three letter code) [5-FAM]-Thr-Asn-Ser-Pro-Arg-Arg-Ala-Arg-Ser-Val-Ala-[Lys(Dnp)]-amide Modifications Where 5-FAM is 5-Carboxyfluorescein and Dnp is 2,4-dinitrophenyl- Purity >95% Storage -20°C References Dhama et al., (2020). Coronavirus Disease 2019–COVID-19. Clin Microbiol Rev. 33(4): e00028-20. PMID: 32580969
Gheblawi et al., (2020). Angiotensin-Converting Enzyme 2: SARS-CoV-2 Receptor and Regulator of the Renin-Angiotensin System. Circ Res. 126(10): PMID: 32264791
O’Leary et al., (2021). Unpacking Pandora from Its Box: Deciphering the Molecular Basis of the SARS-CoV-2 Coronavirus. Int J Mol Sci. 22(1): 386. PMID: 33396557
Manufactured in: United Kingdom Fluorescence resonance energy transfer (FRET) peptide substrate derived from SARS-CoV-2 spike (S) S1/S2 site. This FRET peptide exhibits internal fluorescence quenching when intact, however hydrolysis of the peptide between the donor/acceptor pair generates fluorescence, enabling the quantitative measure of enzymatic activity. The S1/S2 site of SARS-CoV-2 S is efficiently cleaved by a wide range of proteases including furin.
SARS-CoV-2 Spike (S) protein is one of the four essential structural proteins from the coronavirus SARS-CoV-2. S protein is a large, class I viral transmembrane protein essential for viral entry into the cell via binding to the host angiotensin-converting enzyme 2 (ACE2) receptor. S assembles as a trimer on the surface of the virion, giving it its distinctive “corona” or crown-like appearance. The ectodomains of S proteins are divided into two subunits, S1 and S2. S1 helps in host receptor binding and is further divided into two subdomains: N-terminal domain (NTD) and C-terminal domain (CTD), both of which act as receptor-binding domains. The S1 CTD contains the receptor-binding motif (RBM). The S2 subunit accounts for fusion.
Peptide contains an N-terminal 5-carboxyfluorescein (5-FAM), a widely used green fluorescent tag and a 2, 4-dinitrophenyl (Dnp) quencher.