[5-FAM]/[Lys(Dabcyl)]-CoV Main Protease (Mpro) Substrate
[5-FAM]-AVLQSGFR-[Lys(Dabcyl)]-K-amide
Description
Application Data
Description
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FRET peptide substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). Fluorescence can be detected upon cleavage of the peptide by SARS-CoV Mpro
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1101508 Molecular Weight 1740.8 Sequence (one letter code) [5-FAM]-AVLQSGFR-[Lys(Dabcyl)]-K-amide
Sequence (three letter code) [5-FAM]-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-[Lys(Dabcyl)]-Lys-NH2
Aliase Coronavirus, SARS-CoV Purity >95% Storage -20°C References Yang et al (2005) Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biol. 3(10) e324 www.ncbi.nlm.nih.gov/pubmed/16128623
Zhao et al (2008). Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1. J. Virol. 82(17) 8647 www.ncbi.nlm.nih.gov/pubmed/18562531
Manufactured in: United Kingdom FRET peptide substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The substrate sequence is derived from residues P4–P5′ of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. SARS-CoV Mpro is a key antiviral target.
This peptide contains an N-terminal 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag and the Dabcyl fluorescence quencher. When the peptide is intact, fluorescence is undetectable due to the proximity of the Dabcyl quencher to the 5-FAM fluorophore. However upon cleavage of the peptide by SARS-CoV Mpro, the 5-FAM group and the Dabcyl quencher are separated and fluorescence can be detected. This therefore represents a useful tool for investigating SARS-CoV Mpro activity.
[5-FAM]/[Lys(Dabcyl)]-CoV Main Protease (Mpro) Substrate
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