EB1 is a highly potent and efficient cell penetrating peptide (CPP). It contains 5-carboxyfluorescein (5-FAM), a widely used green fluorescent tag.
Catalogue number crb1100284 Molecular Weight 3458.07 Sequence (one letter code) [5-FAM]-LIRLWSHLIHIWFQNRRLKWKKK-amide Sequence (three letter code) [5-FAM]-Leu-Ile-Arg-Leu-Trp-Ser-His-Leu-Ile-His-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Leu-Lys-Trp-Lys-Lys-Lys-NH2 Molecular Weight 3458.07 Purity >95% References
Leśniewska et al (2014) Peptide aptamers define distinct EB1- and EB3-binding motifs and interfere with microtubule dynamics. Mol. Biol. Cell 25(7) 1025 PMID: 24478452
EB1 is a penetratin analogue that was synthesised to be an endosomolytic cell penetrating peptide (CPP). Certain amino acids in the penetratin sequence were replaced with histidine to encourage formation of an alpha helix upon protonation in the acidic endosomes. As a CPP, EB1 has been shown to form a strong interaction with the phospholipid bilayer during insertion with rapid cellular uptake, there is a moderate amount of cell leakage and no significant cytotoxicity. EB1 is provided here as a C-terminal amide with a N-terminal 5-carboxyfluorescein (5-FAM), a widely used green fluorescent tag often preferred due to its high stability; absorbance 492 (nm), 518 emission (nm).