RAF1 peptide
Description
Application Data
Description
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Antigenic peptide corresponding to region in human Raf1 protein
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1200405 Antibody RAF1 peptide Antigen Peptide RAF1 peptide Protein ID UniProtKB - P04049 Aliases Raf-1 Proto-Oncogene, Serine/Threonine Kinase, Oncogene RAF1, CRAF, C-Raf Cross-Reactivity Human Target Protein Species Human Storage Stabilisers -20°C Specificity Protein Storage -20°C Citations Buscaino, A., White, S., Houston, D., Lejeune, E., Simmer, F., de Lima Alves, F., Diyora, P., Urano, T., Bayne, E., Rappsilber, J. and Allshire, R. (2012). Raf1 Is a DCAF for the Rik1 DDB1-Like Protein and Has Separable Roles in siRNA Generation and Chromatin Modification. PLoS Genetics, 8(2), e1002499. PMID: 22319459
References Buscaino, A., White, S., Houston, D., Lejeune, E., Simmer, F., de Lima Alves, F., Diyora, P., Urano, T., Bayne, E., Rappsilber, J. and Allshire, R. (2012). Raf1 Is a DCAF for the Rik1 DDB1-Like Protein and Has Separable Roles in siRNA Generation and Chromatin Modification. PLoS Genetics, 8(2), e1002499. PMID: 22319459
Raf1 forms part of the histone H3K9 methyltransferase complex CLRC, along with Clr4, Raf2, Cul4, and Rik1 and is required for heterochromatin formation. CLRC mediates H3K9 methylation, siRNA production and also displays E3-ubiquitin ligase activity in vitro.
Raf1 is a DCAF (DDB1 and CUL4 associated factor) for CLRC. DCAFs act as substrate receptors for E3 ligases and may couple ubiquitination with histone methylation. Raf1 plays an essential role in coupling H3K9 methylation and siRNA production.