Phospho-FoxO1 (pS218) peptide
Description
Application Data
Description
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Antigenic peptide of FoxO1 transcriptional regulator with phosphorylated serine 218 residue
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1200468 Antibody Phospho-FoxO1 (pS218) peptide Antigen Peptide Phospho-FoxO1 (pS218) peptide Protein ID UniProtKB - Q12778 Cross-Reactivity Human, rat, mouse. May also recognise the equivalent serine in FoxO3/4/6 Target Protein Species Human, rat, mouse. May also recognise the equivalent serine in FoxO3/4/6 Storage Stabilisers -20°C Specificity Protein Storage -20°C Citations Riddell, M., Nakayama, A., Hikita, T., Mirzapourshafiyi, F., Kawamura, T., Pasha, A., Li, M., Masuzawa, M., Looso, M., Steinbacher, T., Ebnet, K., Potente, M., Hirose, T., Ohno, S., Fleming, I., Gattenlöhner, S., Aung, P., Phung, T., Yamasaki, O., Yanagi, T., Umemura, H. and Nakayama, M. (2018). aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability. Nature Comm, 9(1). PMID: 30559384
Wilhelm, K., Happel, K., Eelen, G., Schoors, S., Oellerich, M., Lim, R., Zimmermann, B., Aspalter, I., Franco, C., Boettger, T., Braun, T., Fruttiger, M., Rajewsky, K., Keller, C., Brüning, J., Gerhardt, H., Carmeliet, P. and Potente, M. (2016). FOXO1 couples metabolic activity and growth state in the vascular endothelium. Nature, 529(7585), 216-220. PMID: 26735015
References Riddell, M., Nakayama, A., Hikita, T., Mirzapourshafiyi, F., Kawamura, T., Pasha, A., Li, M., Masuzawa, M., Looso, M., Steinbacher, T., Ebnet, K., Potente, M., Hirose, T., Ohno, S., Fleming, I., Gattenlöhner, S., Aung, P., Phung, T., Yamasaki, O., Yanagi, T., Umemura, H. and Nakayama, M. (2018). aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability. Nature Comm, 9(1). PMID: 30559384
Wilhelm, K., Happel, K., Eelen, G., Schoors, S., Oellerich, M., Lim, R., Zimmermann, B., Aspalter, I., Franco, C., Boettger, T., Braun, T., Fruttiger, M., Rajewsky, K., Keller, C., Brüning, J., Gerhardt, H., Carmeliet, P. and Potente, M. (2016). FOXO1 couples metabolic activity and growth state in the vascular endothelium. Nature, 529(7585), 216-220. PMID: 26735015
Forkhead box O1 (FoxO1) is a transcriptional regulator of cell growth and proliferation controlled by a receptor tyrosine kinase signalling pathway (the vascular endothelial growth factor A (VEGF-A)/phosphatidylinositol-3-OH kinase (PI3K)/protein kinase B (PKB/Akt) pathway). FoxO1 is phosphorylated in the DNA binding domain at serine 218 by aPKCλ. FoxO1 Ser218 phosphorylation and aPKC expression correlate with poor patient prognosis in various cancers.
VEGF/PI3K/Akt signalling results in FoxO1 cytoplasmic localisation and its inactivation. Cytoplasmically localised FoxO1 is associated with cellular myelocytomatosis (c-Myc) expression and endothelial cell (EC) proliferation. Abnormal atypical protein kinase C (aPKC)/FoxO1/c-Myc signalling contributes to excessive EC proliferation in angiosarcoma and when constitutively active, FoxO1 leads to lethality due to heart defects.