Recognises WT1 in Western blots and immunohistochemistry with a strong signal and low background noise. WT1 is a zinc finger transcription factor which plays a key role in kidney development
Notochord injury triggers local and sustained wt1b expression. Immunohistochemistry of the injured area with anti-GFP and anti-WT1 antibodies. n > 10; experimental replicates = 5. Scale bar: 20 mm. dpf = days post fertilization; hpi = hours post injury; H and E = haematoxylin and eosin.
Image provided courtesy of Dr. Elizabeth Patton Laboratory. University of Edinburgh.
|Antigen Peptide||KLH conjugated synthetic peptide crb1200511e|
|Protein ID||UniProtKB - Q1L884|
|Aliases||WT1 transcription factor, Wilms tumor suppressor protein 1b|
|Cross-Reactivity||Danio rerio (Zebrafish)|
|Target Protein Species||Danio rerio (Zebrafish)|
|Validation||1:1000 (ELISA), 1:25000 (IHC)|
|Target||Wt1a and Wt1b|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Disease Area||Tumour, tissue regeneration|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Lopez-Baez et al (2018). Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair. Elife. pii: e30657. PMID: 29405914
Perner et al., (2007). The Wilms tumor genes wt1a and wt1b control different steps during formation of the zebrafish pronephros. Dev. Biol. 309(1): 87. PMID: 17651719
Hastie (2017). Wilms’ tumour 1 (WT1) in development, homeostasis and disease. Development. 144(16):2862. PMID: 28811308
Wilms tumour 1 (WT1) is a zinc finger transcription factor that plays an important role in the development of several mesodermal tissues including: kidneys; gonads; coronary vasculature; cells within the liver; part of the intestine, and adipose tissue.
WT1 is expressed during development, with levels within the developing kidney seen to increase as development proceeds. Postnatally WT1 expression is down-regulated, but upregulated in response to tissue damage in both mice and zebrafish. WT1 has an early role in kidney development and a later role in maintaining kidney function. Mutations in WT1 lead to Wilms tumour, a paediatric kidney cancer, as well as developmental anomalies in the urogenital tract. In zebrafish, two paralogous wt1 genes exist, wt1a and wt1b. The wt1 genes are expressed in a similar and overlapping but not identical pattern.
This antibody is raised using an HPLC-purified peptide from a zinc finger domain of zebrafish WT1, an epitope found in both WT1a and WT1b.