Anti-V5 Epitope Tag antibody
Recognises the V5-tag present on V5-tagged recombinant proteins
Catalogue number crb2005083 Antibody Anti-V5 Epitope Tag antibody Antigen Peptide KLH conjugated synthetic peptide crb1200007e Aliases V5 Tag Host Species Rabbit Antibody Type Polyclonal Concentration 1mg/ml Validation 1:2000 (WB), 1:1000 (ELISA) Target V5 Epitope Tag Storage Stabilisers This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. Specificity Protein Tag Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. References
Cotter, K., Capecci, J., Sennoune, S., Huss, M., Maier, M., Martinez-Zaguilan, R. and Forgac, M. (2014). Activity of Plasma Membrane V-ATPases Is Critical for the Invasion of MDA-MB231 Breast Cancer Cells. J Biol Chem, 290(6), 3680-3692. PMID: 25505184
Ranawakage, D., Takada, T. and Kamachi, Y. (2019). HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions. Sci Rep, 9(1). PMID: 31053795
Anti-V5 Epitope tag antibody corresponding to the V5 epitope sequence GKPIPNPLLGLDST. The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus (of the simian virus 5 (SV5) family) and is extensively used as a general epitope tag in expression vectors. This antibody detects and purifies V5 tagged recombinant proteins without the need for a protein-specific antibody.