Anti-T2A antibody

  • Description

  • Application Data

Description

Recognises the 2A peptide from foot-and-mouth disease virus and can detect 2A tagged recombinant proteins

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Application Data

Catalogue number crb2005269
Antibody Anti-T2A antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200519e
Aliases T2A, Thosea asigna virus 2A
Cross-Reactivity T2A tag
Target Protein Species T2A tag
Host Species Rabbit
Antibody Type Polyclonal
Concentration 0.5mg/ml Glycine, 0.2mg/ml TEA (Rb L02T), 0.5mg/ml Glycine (Rb L02G)
Validation 1:1000 (ELISA)
Target T2A
Storage Stabilisers This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
Specificity T2A
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
Citations

Ferreira, A.C.F., Szeto, A.C.H., Heycock, M.W.D. et al. RORα is a critical checkpoint for T cell and ILC2 commitment in the embryonic thymus. Nat Immunol (2021). https://doi.org/10.1038/s41590-020-00833-w

References

Hofacre et al., (2018). Efficient Therapeutic Protein Expression Using Retroviral Replicating Vector with 2A Peptide in Cancer Models. Hum Gen Ther, 29(4), 437-451. PMID: 29216761

 

Sun et al., (2017). Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system. PLOS ONE, 12(3), e0174804. PMID: 28358924

Data Sheet Data Sheet Data Sheet Data Sheet Material Safety Data Sheet (MSDS)

2A peptide-linked polycistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequences, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel “cleavage” event within the 2A peptide sequence.

2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus). The 2A peptide acts through ribosomal skipping to allow for the encoding of polyproteins which can dissociate into individual proteins upon translation. Anti-T2A antibody recognises 2A tagged recombinant proteins and is an excellent tool for researchers using 2A peptide based expression systems.

Anti-T2A antibody

Cat No.Pack SizePriceQty.
20µg£110.00
0.1mg£220.00
20µg£110.00
0.1mg£220.00
Bulk Quote

A high quality antibody against the T2A peptide

Andrew McKenzie and his team from the MRC Laboratory of Molecular Biology (LMB) enlisted the expertise of the team at Cambridge Research Biochemicals (CRB) to produce a highly specific antibody to the 2A peptide sequence. 2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus) and is used in cloning vectors. The team at MRC…

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