Anti-T2A antibody
Description
Application Data
Description
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Recognises the 2A peptide from foot-and-mouth disease virus and can detect 2A tagged recombinant proteins
Application Data
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Data Sheet Data Sheet Data Sheet Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2005269 Antibody Anti-T2A antibody Antigen Peptide KLH conjugated synthetic peptide crb1200519 Aliases T2A, Thosea asigna virus 2A Cross-Reactivity T2A tag Host Species Rabbit Antibody Type Polyclonal Concentration 0.5 mg/ml Glycine, 0.2 mg/ml TEA (Rb L02T), 0.5 mg/ml Glycine (Rb L02G) Validation 1:1000 (ELISA) Target T2A Specificity T2A Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/thaw cycles. Citations Hofacre et al., (2018). Efficient Therapeutic Protein Expression Using Retroviral Replicating Vector with 2A Peptide in Cancer Models. Hum Gen Ther, 29(4), 437-451. PMID: 29216761
Sun et al., (2017). Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system. PLOS ONE, 12(3), e0174804. PMID: 28358924
References Hofacre et al., (2018). Efficient Therapeutic Protein Expression Using Retroviral Replicating Vector with 2A Peptide in Cancer Models. Hum Gen Ther, 29(4), 437-451. PMID: 29216761
Sun et al., (2017). Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system. PLOS ONE, 12(3), e0174804. PMID: 28358924
2A peptide-linked polycistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequences, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel “cleavage” event within the 2A peptide sequence.
2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus). The 2A peptide acts through ribosomal skipping to allow for the encoding of polyproteins which can dissociate into individual proteins upon translation. Anti-T2A antibody recognises 2A tagged recombinant proteins and is an excellent tool for researchers using 2A peptide based expression systems.