Recognises the 2A peptide from foot-and-mouth disease virus and can detect 2A tagged recombinant proteins
Catalogue number crb2005269 Antibody Anti-T2A antibody Antigen Peptide KLH conjugated synthetic peptide crb1200519e Aliases T2A, Thosea asigna virus 2A Cross-Reactivity T2A tag Target Protein Species T2A tag Host Species Rabbit Antibody Type Polyclonal Concentration 0.5mg/ml Glycine, 0.2mg/ml TEA (Rb L02T), 0.5mg/ml Glycine (Rb L02G) Validation 1:1000 (ELISA) Target T2A Storage Stabilisers This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. Specificity T2A Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. Citations
Ferreira, A.C.F., Szeto, A.C.H., Heycock, M.W.D. et al. RORα is a critical checkpoint for T cell and ILC2 commitment in the embryonic thymus. Nat Immunol (2021). https://doi.org/10.1038/s41590-020-00833-w
Hofacre et al., (2018). Efficient Therapeutic Protein Expression Using Retroviral Replicating Vector with 2A Peptide in Cancer Models. Hum Gen Ther, 29(4), 437-451. PMID: 29216761
Sun et al., (2017). Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system. PLOS ONE, 12(3), e0174804. PMID: 28358924
2A peptide-linked polycistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequences, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel “cleavage” event within the 2A peptide sequence.
2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus). The 2A peptide acts through ribosomal skipping to allow for the encoding of polyproteins which can dissociate into individual proteins upon translation. Anti-T2A antibody recognises 2A tagged recombinant proteins and is an excellent tool for researchers using 2A peptide based expression systems.
Andrew McKenzie and his team from the MRC Laboratory of Molecular Biology (LMB) enlisted the expertise of the team at Cambridge Research Biochemicals (CRB) to produce a highly specific antibody to the 2A peptide sequence. 2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus) and is used in cloning vectors. The team at MRC…