Anti-SARS-CoV-2 Spike protein antibody
Description
Application Data
Description
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Recognises SARS-CoV-2 Spike protein polybasic cleavage site
Application Data
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Anti-SARS-CoV-2 Spike protein antibody (crb2005280) K98
A. SDS PAGE and Coomassie stain and B. western blot analysis of 500ng Spike S1 Recombinant protein.
Lane 1: SARS-Cov-2 Spike S1 protein
Lane 2: SARS-Cov Spike S1 protein
Lane 3: MERS Spike S1 protein
Expected sizes: SARS-Cov-2 spike S1
| Catalogue number | crb2005280 |
|---|---|
| Antibody | Anti-SARS-CoV-2 Spike protein antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200514e |
| Aliases | Coronavirus, SARS-CoV-2, Covid-19 |
| Cross-Reactivity | Virus |
| Target Protein Species | Virus |
| Host Species | Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 0.5 mg/ml |
| Target | SARS-CoV-2 Spike protein |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Specificity | Recognises SARS-CoV-2 Spike protein polybasic cleavage site |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Byrnes et al., (2020). Competitive SARS-CoV-2 Serology Reveals Most Antibodies Targeting the Spike Receptor-Binding Domain Compete for ACE2 Binding. mSphere. 5(5): e00802-20. PMID: 32938700
Wang et al., (2020). Molecular simulation of SARS-CoV-2 spike protein binding to pangolin ACE2 or human ACE2 natural variants reveals altered susceptibility to infection. J Gen Virol. 101(9): 921. PMID: 32538738 |
The SARS-CoV2 is a novel coronavirus identified as the cause of the coronavirus disease 2019 (COVID-19) pandemic that began in Wuhan, China in 2019. SARS-CoV-2 can cause severe diseases such as pneumonia, acute respiratory distress syndrome (ARDS), sepsis, septic shock or multiple organ failure, particularly among the elderly and immunocompromised patients. SARS-CoV-2 virus particles are round, elliptic and often pleomorphic in shape and a diameter of approximately 60–140 nm. The SARS-CoV2 genome encodes four essential structural proteins including the spike (S) glycoprotein, nucleocapsid (N) protein, membrane (M) protein, and the envelope (E) protein, and also several accessory proteins.
The SARS-CoV-2 spike protein is present on the outside of the virus particles and is able to bind to angiotensin-converting enzyme II (ACE2) present on the host cells. The C-terminal receptor binding domain (RBD) of the spike protein binds to the N-terminal peptidase M2 domain of ACE2. This receptor binding results in internalisation of the virus-receptor complex and is therefore the mechanism of entry of SARS-CoV-2 into host cells.