Anti-ROBO1 antibody
Description
Application Data
Description
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Antibody raised against ROBO1; acts as receptor for SLIT1 and SLIT2, mediating cellular response to molecular guidance cues in cellular migration and axon navigation.
Application Data
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Material Safety Data Sheet (MSDS)Catalogue number crb2005658 Antibody Anti-ROBO1 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200867e Protein ID Q9Y6N7 ROBO1 Aliases Roundabout Homologue 1, Deleted in U twenty twenty, H-Robo-1, DUTT1 Cross-Reactivity Mouse, Rat Target Protein Species Mouse, Rat Host Species Rabbit Antibody Type Polyclonal Concentration 1mg/ml Glycine (R1G), 2mg/ml Glycine (R2G) Target ROBO1 Storage Stabilisers The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. Family ROBO family Storage The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. References Kidd, T., et al. (1998). Roundabout Controls Axon Crossing of the CNS Midline and Defines a Novel Subfamily of Evolutionarily Conserved Guidance Receptors. Cell, 92(2), pp.205–215.
Lopez-Bendito, G., et al. (2007). Robo1 and Robo2 Cooperate to Control the Guidance of Major Axonal Tracts in the Mammalian Forebrain. Journal of Neuroscience, 27(13), pp.3395–3407.
Mertsch, S., et al. (2007). Slit2 involvement in glioma cell migration is mediated by Robo1 receptor. Journal of Neuro-Oncology, 87(1), pp.1–7.
Roundabout homologue 1 (Robo1) and Roundabout homologue 2 (Robo2) are receptors which cooperate to mediate the function of Slit proteins, used in guiding major forebrain projections during neuronal development. Robo1 is an axon guidance receptor and cell adhesion receptor, necessary in stimulating neurite outgrowths from Robo-positive neurons. Deficiencies in Robo1 can cause prominent axon pathfinding errors during neuronal cell migration.
Robo1 is activated by the Slit family of proteins, this results in a repulsive effect on glioma cell guidance in the developing brain. In bilaterally symmetrical nervous systems seen in vertebrae, the nervous system midline structures establish the partition between the two halves; Robo1 is essential in the crossing of the central nervous system midline, appearing as the controlling factor in midline crossing of axons.
Robo1 and Robo2 are used in separate, specialised processes in post midline crossing axons. Robo1 is needed to prevent axons from stalling immediately post crossing, whereas Robo2 is required for axons to project away from the floor plate and into the lateral funiculus.