Anti-RAMP3 antibody corresponding to a region within the human RAMP3 C-terminal protein sequence.
Catalogue number crb2005161 Antibody Anti-RAMP3 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200419e Protein ID UniProtKB - O60896 Cross-Reactivity Human Target Protein Species Human Host Species Rabbit Antibody Type Polyclonal Concentration 0.22 mg/ml Validation 1:1000 (ELISA), Target RAMP3 Storage Stabilisers This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. Family RAMP family Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. References
Hay and Pioszak, (2015). RAMPs (Receptor-Activity Modifying Proteins): New Insights and Roles. Annu. Rev. Pharmacol. Toxicol. 56: 469 PMID: 26514202
Anti-RAMP3 antibody corresponds to a region within the human RAMP3 C-terminal protein sequence. RAMP3 is a receptor activity-modifying protein and can interact with G-protein coupled receptors (GPCRs) to alter their function. When in complex with the calcitonin like-receptor (CLR), RAMP chaperones CLR to the surface of the cell where it forms an adrenomedullin2 (AM2) receptor specific for the peptide hormone adrenomedullin. Conversely the calcitonin receptor (CTR) can translocate to the cell surface without RAMP, however RAMP requires CTR to reach the cell surface. It is evident through the use of screening that RAMP requires an interaction with a molecule to translocate to the cell surface.
RAMP3 contains a single transmembrane spanning domain, an extracellular N-terminal domain and multiple glycosylation sites. Within the C-terminus two basic residues serve as an endoplasmic retrieval signal. In contrast with Human RAMP1 and 2, Human RAMP3 contains a type-1 PDZ1 recognition site which interacts with Na+/H+ exchange regulatory factor (NHERF) or N-ethylmaleimide-sensitive factor (NSF). This interaction effects GPCR function through altering receptor internalization and recycling.