Recognises RAMP1, a receptor accessory protein in Western blots and immunohistochemistry with a strong signal and low background noise
Immunofluorescence image of Human Embryonic Kidney cells (HEK293S) transiently transfected with either pcDNA3.1 alone or HA-CLR and RAMP1 for 48h
|Antigen Peptide||KLH conjugated synthetic peptide crb120320e|
|Protein ID||UniProtKB - O60894|
|Cross-Reactivity||Human, Rat, Mouse|
|Target Protein Species||Human, Rat, Mouse|
|Validation||1:200 (WB), 1:100, 1:500, 1:1000 (ELISA), 1:500 (IF)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Li et al., (2014). Deficiency of RAMP1 Attenuates Antigen-Induced Airway Hyperresponsiveness in Mice. PLoS ONE. 9(7): e102356. PMID: 25010197
Suekane et al., (2019). CGRP-CRLR/RAMP1 signal is important for stress-induced hematopoiesis. Sci Rep. 9(1). PMID: 30674976
Receptor activity-modifying protein 1 (RAMP1) is a receptor accessory protein specifically expressed in hematopoietic progenitor cells. RAMP1 associates with G-protein coupled receptors (GPCRs) to alter ligand specificity and mediate receptor trafficking. RAMP1 forms a receptor complex with calcitonin receptor-like receptor (CRLR) known as the calcitonin gene-related protein (CGRP) receptor.
RAMPs also associate with other family B G-protein coupled receptors including the vasoactive intestinal polypeptide/pituitary AC-activating peptide 1 (VPAC1) receptor and the glucagon receptor (GCGR) where they modulate ligand and G-protein selection.