Anti-POLDIP3 antibody
Description
Application Data
Description
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An antibody raised against polymerase delta-interacting protein 3 (POLDIP3); has a role in transcription and translation. POLDIP3 is a critical regulator of disassembling R-loop formations and activating the DNA damage checkpoint.
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2005743 Antibody Anti-POLDIP3 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200952 Protein ID UniProtKB - Q9BY77 (Human) Aliases Polymerase delta-interacting protein 3, 46 kDa DNA polymerase delta interaction protein (p46), S6K1 Aly/REF-like target (SKAR) Cross-Reactivity Human Host Species Rabbit Antibody Type Polyclonal Concentration 2.0 mg/ml Glycine Target Polymerase delta-interacting protein 3 Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/thaw cycles. References Folco et al., (2012). The proteins PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export. PLoS One. 7(8): e43804. doi: 10.1371/journal.pone.0043804.
Singh et al., (2022). POLDIP3: At the Crossroad of RNA and DNA Metabolism. Genes. 13(11): 1921. doi: 10.3390/genes13111921.
POLDIP3 is preferentially associated with CBC-bound spliced mRNA-protein complexes during the initial round of mRNA translation. POLDIP3 is deposited at the exon junction during pre-mRNA splicing. It becomes part of the exon junction complex (EJC), which then facilitates the recruitment of S6K1 to the spliced RNA to boost the translation efficiency of the spliced mRNA. In addition, POLDIP3 is involved in nuclear mRNA export mediated by association with the TREX complex.
POLDIP3 interacts with DNA polymerase Pol δ and PCNA depending on its modifications. This creates a distinct regulatory role for POLDIP3 during DNA synthesis.