Anti-phosphorylated NR2B (pS1303) antibody 50µg
Description
Application Data
Description
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Recognises the NR2B subunit of NMDA receptors phosphorylated at serine residue 1303
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2005002e Antibody Anti-phosphorylated NR2B (pS1303) antibody 50µg Antigen Peptide KLH conjugated synthetic peptide crb1200273e Protein ID UniProtKB - Q00960 Aliases GRIN2B, GluN2B, MRD6, NMDAR2B, NR2B, hNR3, EIEE27, glutamate ionotropic receptor NMDA type subunit 2B, NMDA NR2B Subunit Cross-Reactivity (predicted Human, Mouse) Target Protein Species (predicted Human, Mouse) Host Species Anti-Rabbit Antibody Type Polyclonal Label Unconjugated Concentration 1mg/ml Validation WB/ELISA/IHC Target phosphorylated NR2B (pS1303) Storage Stabilisers This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. Family glutamate-gated ion channel family. Disease Area Neurodegenerative diseases Specificity PTM - Phospho ser 1303 Post-translational Modification Phosphorylation of Serine 1303 Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. Citations Li, Y., Sun, W., Han, S., Li, J., Ding, S., Wang, W. and Yin, Y. (2016). IGF-1-Involved Negative Feedback of NR2B NMDA Subunits Protects Cultured Hippocampal Neurons Against NMDA-Induced Excitotoxicity. Mol Neurobiol, 54(1), 684-696. PMID: 26758454
Liao, G. Y., Wagner. D. A., Hsu, M. H., Leonard, J. P. (2001). Evidence for direct protein kinase-C mediated modulation of N-methyl-D-aspartate receptor current. Mol Pharmacol, 59(5), 960-964. PMID: 11306676
Wang, Y., Han, S., Han, R., Su, Y. and Li, J. (2017). Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain and Behav, 7(7), e00734. PMID: 28729940
References Li, Y., Sun, W., Han, S., Li, J., Ding, S., Wang, W. and Yin, Y. (2016). IGF-1-Involved Negative Feedback of NR2B NMDA Subunits Protects Cultured Hippocampal Neurons Against NMDA-Induced Excitotoxicity. Mol Neurobiol, 54(1), 684-696. PMID: 26758454
Liao, G. Y., Wagner. D. A., Hsu, M. H., Leonard, J. P. (2001). Evidence for direct protein kinase-C mediated modulation of N-methyl-D-aspartate receptor current. Mol Pharmacol, 59(5), 960-964. PMID: 11306676
Wang, Y., Han, S., Han, R., Su, Y. and Li, J. (2017). Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain and Behav, 7(7), e00734. PMID: 28729940
N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ionotropic glutamate receptors that mediate excitatory synaptic transmission and are important for many aspects of nervous system function including: synaptic plasticity; learning and memory; neuronal development and circuit formation. NMDARs have also been implicated in various neuronal disorders. NMDARs are heteromers consisting of an obligate NR1 and most commonly one or two kinds of NR2 subunits or occasionally NR3 subunits.
NR2B is the predominant subunit found in the postnatal brain, however over time NRA2 levels increase and eventually outnumber NR2B subunits in a process known as the NR2B-NR2A developmental switch. The greater ratios of the NR2B subunit in post-natal brains leads to NMDA receptors which remain open longer compared to those with more NR2A subunits, and may be related to the greater memory abilities in the immediate postnatal period compared to late in life.
The phosphorylation of NR2B is closely linked to its transport and the phosphorylation of NR2B at Ser1303 is an initial step of NMDA‐induced excitotoxicity. NR2B is phosphorylated at Ser1303 by protein kinase C (PKC) and is dephosphorylated by protein phosphatase 1 (PP1).
The involvement of NMDAR in the central nervous system (CNS) has become a focus area for neurodegenerative diseases such as Alzheimer’s disease, epilepsy and ischemic neuronal cell death.