Anti-phosphorylated NR2A (pS1232) antibody
Recognises the NR2A subunit of NMDA receptor phosphorylated on ser1232
Western blot analysis of rat brain preparation.
Lane 1: Rat brain preparation (20µg).
Secondary: Goat anti-rabbit IgG conjugated to HRP 1:2000.
|Antibody||Anti-phosphorylated NR2A (pS1232) antibody|
|Antigen Peptide||KLH conjugated synthetic peptide crb1200271e|
|Protein ID||UniProtKB - P35436|
|Aliases||GRIN2A, GluN2A, NMDAR2A, NR2A, EIEE27, glutamate ionotropic receptor NMDA type subunit 2A/ NMDA NR2A Subunit|
|Target Protein Species||Mouse, Rat|
|Validation||1:1000 (WB), 1:1000 (ELISA)|
|Target||phosphorylated NR2A (pS1232)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Family||Glutamate-gated ion channel family.|
|Super Family||Ionotropic receptor|
|Disease Area||Neurodegenerative diseases|
|Specificity||PTM - Phosphorylated serine 1232|
|Post-translational Modification||Phosphorylation of Serine 1232|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Dong, L., Chen, J., Li, F., Gao, F., Wu, J., Miao, Y. and Wang, Z. (2013). Enhanced Expression of NR2B Subunits of NMDA Receptors in the Inherited Glaucomatous DBA/2J Mouse Retina. Neural Plast, 2013, 1-7. PMID: 24175101
Li, B., Sun, M., Zhang, L., Takahashi, S., Ma, W., Vinade, L., Kulkarni, A., Brady, R. and Pant, H. (2001). Regulation of NMDA receptors by cyclin-dependent kinase-5. PNAS, 98(22), 12742-12747. PMID: 11675505
N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ionotropic glutamate receptors that mediate excitatory synaptic transmission and play important roles in many aspects of nervous system function including: synaptic plasticity; learning and memory; neuronal development and circuit formation. In addition NMDARs have also been implicated in various neuronal disorders. NMDARs are heteromers consisting of an obligate NR1 subunit and most commonly one or two kinds of NR2 subunits or occasionally NR3 subunits.
NMDARs are phosphorylated by both serine/threonine and tyrosine kinases. Cyclin dependent kinase-5 (Cdk5) associates with and phosphorylates NR2A subunits at the serine residue at position 1232. Phosphorylation of NR2A at Ser1232 (p-NR2AS1232) by Cdk5/p35 may contribute to neuronal cell death.
The involvement of NMDAR in the central nervous system (CNS) has become a focus area for neurodegenerative diseases such as Alzheimer’s disease, epilepsy and ischemic neuronal cell death.