Anti-phospho-HtrA2 (pS400) antibody

  • Description

  • Application Data

Description

An antibody raised against HtrA2 phosphorylated at serine 400 (S400), a mitochondrial serine protease chaperone associated with neurodegenerative disorders.

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Application Data

Catalogue number crb2005320
Antibody Anti-phospho-HtrA2 (pS400) antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200554
Protein ID UniProtKB: O43464
Aliases High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI
Cross-Reactivity Human
Target Protein Species Human
Host Species Anti-Rabbit
Antibody Type Polyclonal
Concentration N18 0.5 mg/ml, N19 1mg/ml
Validation ELISA (1:1000)
Target phosphorylated HtrA2 (pS400)
Storage Stabilisers The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.
Disease Area Cancer, Cell signalling, Metabolism
Specificity Protein
Post-translational Modification Phosphorylation
Storage The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.
Citations

Fitzgerald et al., (2012). Phosphorylation of HtrA2 by cyclin-dependent kinase-5 is important for mitochondrial function. Cell Death Differ.19(2): 257-66. PMID: 21701498.

References

Desideri and Martins, (2012). Mitochondrial Stress Signalling: HTRA2 and Parkinson’s Disease. Int. J. Cell Biol., 607929: 1. DOI: 10.1155/2012/607929.

Fitzgerald et al., (2012). Phosphorylation of HtrA2 by cyclin-dependent kinase-5 is important for mitochondrial function. Cell Death Differ, 19: 257. DOI: 10.1038/cdd.2011.90.

Material Safety Data Sheet (MSDS)

Human High-temperature requirement As (HtrAs) function as dual-protease chaperones in an adenosine 5′-triphosphate (ATP)-independent manner. HtrAs play critical roles in protein quality control and modulating signalling pathways by cleaving or sequestering regulatory proteins.

Mammals express 4 HtrA proteases. The HtrA2 serine protease is essential for mitochondrial homeostasis and programmed cell death. Aberrant HtrA2 activity is associated with neurodegenerative disorders, including Parkinson’s disease. HtrA2 forms a hexameric state with low affinity for substance rates in an inactive state. HtrA2 forms a trimer and binds an activator peptide to each HtrA2 peptomer, revealing the catalytic centre and a significant increase in protease activity.

HtrA2 localises to the mitochondrial intermembrane space. Apoptotic stress leads to HtrA2 localises to the cytoplasm to degrade inhibitor of apoptosis proteins activating the apoptosis cascade. HtrA2 also has neuroprotective effects by maintaining mitochondrial protein homeostasis. Phosphorylation of HtrA2 by cyclin-dependent kinase Cdk5 at S400 is vital to maintain the mitochondrial membrane potential during stress conditions. Missense mutations in HTRA2 genes are found in Parkinson’s disease patients. In particular, mutations adjacent to S400 have been characterised in Parkinson’s patients.

Anti-phospho-HtrA2 (pS400) antibody

Cat No.Pack SizePriceQty.
20µg£170.00
50µg£250.00
20µg£170.00
50µg£250.00
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