Anti-phospho-HtrA2 (pS400) antibody
Description
Application Data
Description
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An antibody raised against HtrA2 phosphorylated at serine 400 (S400), a mitochondrial serine protease chaperone associated with neurodegenerative disorders.
Application Data
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Material Safety Data Sheet (MSDS)Catalogue number crb2005320 Antibody Anti-phospho-HtrA2 (pS400) antibody Antigen Peptide KLH conjugated synthetic peptide crb1200554 Protein ID UniProtKB: O43464 Aliases High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI Cross-Reactivity Human Target Protein Species Human Host Species Anti-Rabbit Antibody Type Polyclonal Concentration N18 0.5 mg/ml, N19 1mg/ml Validation ELISA (1:1000) Target phosphorylated HtrA2 (pS400) Storage Stabilisers The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. Disease Area Cancer, Cell signalling, Metabolism Specificity Protein Post-translational Modification Phosphorylation Storage The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. Citations Fitzgerald et al., (2012). Phosphorylation of HtrA2 by cyclin-dependent kinase-5 is important for mitochondrial function. Cell Death Differ.19(2): 257-66. PMID: 21701498.
References Desideri and Martins, (2012). Mitochondrial Stress Signalling: HTRA2 and Parkinson’s Disease. Int. J. Cell Biol., 607929: 1. DOI: 10.1155/2012/607929.
Fitzgerald et al., (2012). Phosphorylation of HtrA2 by cyclin-dependent kinase-5 is important for mitochondrial function. Cell Death Differ, 19: 257. DOI: 10.1038/cdd.2011.90.
Human High-temperature requirement As (HtrAs) function as dual-protease chaperones in an adenosine 5′-triphosphate (ATP)-independent manner. HtrAs play critical roles in protein quality control and modulating signalling pathways by cleaving or sequestering regulatory proteins.
Mammals express 4 HtrA proteases. The HtrA2 serine protease is essential for mitochondrial homeostasis and programmed cell death. Aberrant HtrA2 activity is associated with neurodegenerative disorders, including Parkinson’s disease. HtrA2 forms a hexameric state with low affinity for substance rates in an inactive state. HtrA2 forms a trimer and binds an activator peptide to each HtrA2 peptomer, revealing the catalytic centre and a significant increase in protease activity.
HtrA2 localises to the mitochondrial intermembrane space. Apoptotic stress leads to HtrA2 localises to the cytoplasm to degrade inhibitor of apoptosis proteins activating the apoptosis cascade. HtrA2 also has neuroprotective effects by maintaining mitochondrial protein homeostasis. Phosphorylation of HtrA2 by cyclin-dependent kinase Cdk5 at S400 is vital to maintain the mitochondrial membrane potential during stress conditions. Missense mutations in HTRA2 genes are found in Parkinson’s disease patients. In particular, mutations adjacent to S400 have been characterised in Parkinson’s patients.
