Anti-Phospho-GluR1 (pS845) antibody
Recognises the GluR1 subunit of AMPAR with a phosphorylated serine at position 845
Catalogue number crb2005048 Antibody Anti-Phospho-GluR1 (pS845) antibody Antigen Peptide Anti-phospho-GluR1 (pS845) antibody Protein ID UniProtKB - P35438 Aliases GluA1 Cross-Reactivity Human, rat, mouse, goat Target Protein Species Human, rat, mouse, goat Host Species Anti-Rabbit Antibody Type Polyclonal Label Unconjugated Concentration 1mg/ml Validation 1:1000 (WB), 1:1000 (ELISA) Target phosphorylated GluR1 (pS845) Storage Stabilisers This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4oC for short term storage and -20oC for long term storage. Avoid repeated freeze/ thaw cycles. Family AMPA receptor Disease Area Neurodegenerative Specificity Protein Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4oC for short term storage and -20oC for long term storage. Avoid repeated freeze/ thaw cycles. References
Khatib, T., Whiting, A., Chisholm, D., Redfern, C., Müller, B. and McCaffery, P. (2019). A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. Mol Neurobiol. PMID: 30972628
Spaethling, J., Le, L. and Meaney, D. (2012). NMDA receptor mediated phosphorylation of GluR1 subunits contributes to the appearance of calcium-permeable AMPA receptors after mechanical stretch injury. Neurobiol Dis, 46(3), 646-654. PMID: 22426393
The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) is an ionotropic transmembrane receptor for glutamate found throughout the central nervous system (CNS). AMPARs are composed of four types of subunits: GluR1/GluA1, GluR2/GluA2, GluR3/GluA3, and GluA4 (GluRA-D2), which combine to form heterotetramers.
Phosphorylation of AMPARs is often implicated in synaptic plasticity and the pathological signalling seen in several disease states. Phosphorylation of GluR1 at serine 845 has been implicated in specific neurological diseases including paediatric seizures. Phosphorylation of the S845 of the GluR1 subunit is controlled by cGKII, a kinase activated by an increase in nitric oxide (NO) levels within the cytosol, and PKA, activated by the calcium dependent production of cAMP. GluR1 S845 is dephosphorylated by the protein phosphatases PP1, PP2A, and CaN (also known as PP2B).