Recognizes integrated stress response (ISR) kinase, PERK
Western blot analysis of fly heads (three per lane) from indicated genotypes. Blotting for actin provided the loading control. Note the increased level of PERK-WT immunoreactivity in GADD34-rescued flies.This reflects the preservation of PERK expressing retinal tissue.
|Antigen Peptide||Drosophila PERK kinase domain recombinant protein|
|Protein ID||UniProtKB - Q9NIV1 (E2AK3_DROME)|
|Aliases||Drosophila PEK (pancreatic eIF2α kinase), CG2087, Drosophila Eukaryotic translation initiation factor 2-alpha kinase (E2AK3)|
|Target Protein Species||Drosophila melanogaster|
|Super Family||eIF2α kinases|
|Disease Area||Unfolded protein response, Wolcott Rallison Syndrome / diabetes mellitus|
|Storage||The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
Malzer et al., (2010). Impaired tissue growth is mediated by checkpoint kinase 1 (CHK1) in the integrated stress response. J Cell Sci. 1: 123(17): 2892. PMID: 20682638
Recognizes Integrated stress response (ISR) kinase, PERK, with a strong signal and low background noise.
When there are a large number of misfolded proteins in the cell a homeostatic mechanism called the unfolded protein response (UPR) is activated which involves restricting translation and increasing chaperone capacity. As part of this response, the ER-resident kinase PERK (eukaryotic translation initiation factor 2α kinase, also known as PEK) phosphorylates the translation initiation factor eIF2α resulting in the attenuation of translation.
PERK activation causes loss of cyclin D1 and G1 cell cycle arrest, negatively regulating cell proliferation. PERK-dependent activation of GSK3β during ER stress has been shown to destabilise p53.