Anti-p21 antibody corresponding to a region of the human p21 protein sequence.
Western blot analysis of whole cell extract of human osteocarcinoma (U-2 OS) (20µg).
Lane 1: U-2 OS cells
Lane 2: U-2 OS cells transfected with siRNA p21 (48h)
Lane 3: U-2 OS cells transfected with RSV-p21 for (48h)
Western blot image provided courtesy of Dr. Sonia Rocha
|Antigen Peptide||KLH conjugated synthetic peptide crb1200312e|
|Protein ID||UniProtKB - P38936|
|Aliases||Cyclin-dependent kinase inhibitor 1, CDKN1, CDK-Interacting Protein 1, CIP1, Wild-Type P53-Activated Fragment 1, WAF1, Melanoma Differentiation Associated Protein 6, MDA6|
|Target Protein Species||Human|
|Validation||1:1000 (WB), 1:1000 (ELISA)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Family||Cyclin-dependent kinase inhibitor|
|Disease Area||Cell Cycle, DNA Damage, Senescence, Apoptosis|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
The multi-functional cyclin-dependent kinase (CDK) inhibitor p21, also known as p21WAF1/Cip1 or CDKN1A functions as a cell cycle inhibitor and anti-proliferative effector. Upon DNA damage and genotoxic stress, the tumour suppressor p53 is activated and induces the expression of p21. p21 has also been implicated in nucleotide excision repair (NER), base excision repair (BER) and DNA translesion synthesis (TLS) by disrupting the proliferating cell nuclear antigen (PCNA) interaction with other DNA repair factors and promoting PCNA degradation.