Recognises Murine leukaemia virus (MLV) Gag cleavage product, p12 in Western blot and immunelocalisation with a strong signal and low background noise
p12 and CA co-localise on mitotic chromatin. HeLa cells synchronised using a double-aphidicolin block were infected with WT Mo-MLV VLPs or mutants carrying p12_mut14 or IN_W390A. 10 h post-infection
|Aliases||murine leukaemia virus (MLV) Gag cleavage product, p12|
|Cross-Reactivity||murine leukaemia virus (MLV)|
|Target Protein Species||murine leukaemia virus (MLV)|
|Storage Stabilisers||The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
|Storage||The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
Wanaguru M, Barry DJ, Benton DJ, O’Reilly NJ, Bishop KN (2018) Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis. PLoS Pathog 14(6): e1007117. https://doi.org/10.1371/journal.ppat.1007117
Brzezinski et al., (2016). Phosphorylation Requirement of Murine Leukemia Virus p12. J Virol. 90(24): 11208. PMID: 27707931
Wight et al., (2014). The N-Terminus of Murine Leukaemia Virus p12 Protein Is Required for Mature Core Stability. PLoS Pathog. 10(10): e1004474. PMID: 25356837
P12 is a cleavage product from the murine leukaemia virus (MLV) Gag protein and is essential for various steps in viral replication and during both early and late stages of murine leukaemia virus (MLV) infection.
The N- and C-terminal regions of p12 are sequentially acting domains, both required for p12 function. The N-terminal domain of p12 binds directly to the MLV capsid protein (CA), thus stabilising the capsid shell and viral core. p12 contains the PPPY late-domain (L-domain), which is essential for recruiting HECT ubiquitin ligases which regulate pathways to ensure efficient budding.