Anti-NR1 4b antibody
Recognises the 4b splice variant of the NMDA receptor subunit NR1
Western blot analysis of rat brain preparation.
Lane 1: Rat brain preparation (10µg).
Secondary: Goat anti-rabbit IgG conjugated to HRP 1:2000.
|Antibody||Anti-NR1 4b antibody|
|Antigen Peptide||KLH conjugated synthetic peptide crb1200277e|
|Protein ID||UniProt P35439 (Rat) , UniProt Q05586 (Human)|
|Aliases||NR1, MRD8, NMDAR1, NMDA Zeta-1, NMD-R1, GluN1, glutamate ionotropic receptor NMDA type subunit 1, NMDA NR1 Subunit|
|Cross-Reactivity||Human, Rat, Mouse|
|Target Protein Species||Human, Rat, Mouse|
|Target||NR1 4b, 4d, 4e 4g|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Family||Glutamate-gated ion channel family.|
|Disease Area||Neurodegenerative diseases|
|Specificity||Rat NR1 4B/D/E/G, Human NR1 Isoform 1/4/6/7|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Donaldson, L. and Beazley-Long, N. (2016). Alternative RNA splicing: contribution to pain and potential therapeutic strategy. Drug Discov Today, 21(11), 1787-1798. PMID: 27329269
Ewald, R. (2009). Cloning and phylogenetic analysis of NMDA receptor subunits NR1, NR2A and NR2B in Xenopus laevis tadpoles. Front Mol Neurosci, 2. PMID: 19826620
N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ionotropic glutamate receptors that mediate excitatory synaptic transmission and play important roles in many aspects of nervous system function including: synaptic plasticity; learning and memory; neuronal development and circuit formation. NMDARs have been implicated in various neuronal disorders. NMDARs are heteromers consisting of an obligate NR1 subunit and most commonly one or two kinds of NR2 subunits or occasionally NR3 subunits.
The NR1 subunit is alternatively spliced yielding eight possible isoforms characterised by the inclusion or deletion of exons 5, 21 and 22. The splice variants are developmentally and spatially regulated and modulate the biophysical properties of NMDARs and alter the trafficking behaviour of NR1.
NR1-4b Splice variants includes exons 5, 21, and 22 and is expressed in the dorsal horn of the spinal cord.
The involvement of NMDAR in the central nervous system (CNS) has become a focus area for neurodegenerative diseases such as Alzheimer’s disease, epilepsy and ischemic neuronal cell death.