Anti-Nocturnin antibody
Description
Application Data
Description
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An antibody against nocturnin, a circadian-regulated 2′-phosphatase with nicotinamide adenine dinucleotide phosphate (NADPH) as its primary substrate.
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2005782 Antibody Anti-Nocturnin antibody Antigen Peptide KLH conjugated synthetic peptide crb1200991 Protein ID UniProtKB - Q9UK39 (Human), Q9ET55 (Rat), O35710 (Mouse) Aliases NOCT Cross-Reactivity Human, Rat, Mouse Host Species Rabbit Antibody Type Polyclonal Concentration 2.0 mg/ml Glycine Target Nocturnin Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/thaw cycles. References Estrella et al., (2019). The metabolites NADP+ and NADPH are the targets of the circadian protein Nocturnin (Curled). Nat Comms, 10(1): 1. doi: 10.1038/s41467-019-10125-z.
Laothamatas et al., (2020). Spatiotemporal regulation of NADP(H) phosphatase Nocturnin and its role in oxidative stress response. PNAS, 117(2): 993. doi: 10.1073/pnas.1913712117.
Nocturnin 2′-phosphatase but was believed to be a deadenylase. Nocturnin is part of the CCR4 family of endonuclease–exonuclease–phosphatase (EEP) enzymes, catalysing the conversion of NADP+ to NAD+ and NADPH to NADH. NOCT has a well-conserved C-terminal EEP domain but lacks a canonical RNA-binding domain and does not contain the leucine-rich repeat domain. It is present in two forms, depending on which initiation codon is used for translation initiation. One form results in the expression of a mitochondrial targeting signal, and nocturnin is transported into the mitochondria. Alternatively, this signal is lacking, and the protein remains in the cytoplasm.
Nocturnin is circadian-regulated with peek expression in the early night hours. It coordinates metabolism that promotes adipogenesis during the circadian clock. Mitochondrial targeting of nocturnin appears to link metabolism and the circadian clock. Deletion in mice leads to resistance to high-fat diet-induced obesity and hepatic steatosis. Nocturnin regulates intracellular NADP(H) levels and the oxidative stress response.