Recognises neuronal nuclear protein, NeuN, a marker for post-mitotic neurones
Immunohistochemistry analysis of paraffin embedded young (3 months) and old (30 months) mouse brain tissue sections.
Immunoreactivity detected in nuclei in the Cortex (Cx) and hippocampus (HP).
Detection: Vector ® NovaRED. Nuclei counterstained with Haemtoxylin
|Antigen Peptide||KLH conjugated synthetic peptide crb1200308e|
|Protein ID||SwissProt - A6NFN3|
|Aliases||RNA Binding Protein Fox-1 Homolog 3, Hexaribonucleotide Binding Protein 3, Neuronal Nuclei Antigen, Fox-1 Homolog C|
|Cross-Reactivity||Human, Rat, Mouse|
|Target Protein Species||Human, Rat, Mouse|
|Validation||1:1000 (ELISA), 1:1000 (IHC)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
V. V. Gusel’nikova and D. E. Korzhevskiy. 2015. NeuN As a Neuronal Nuclear Antigen and Neuron Differentiation Marker. Acta Naturae. 7(2): 42–47. PMID: 26085943
Yun Kyung Hahn, Ruturaj R. Masvekar, Ruqiang Xu, Kurt F. Hauser, and Pamela E. Knapp. 2015. Chronic HIV-1 Tat and HIV Reduce Rbfox3/NeuN: Evidence for Sex-Related Effects. Curr HIV Res; 13(1): 10–20. PMID: 25760045
Neuronal nuclear protein (NeuN) antibody recognises the RNA-binding, feminising locus on X-3 (Rbfox3), a member of the larger mammalian Fox1 family of RNA binding proteins.
Fox1 proteins recognise a unique UGCAUG mRNA motif to regulate neuro-specific alternative splicing and help control neuronal differentiation and central nervous system (CNS) development. In addition, Rbfox3 is also involved in positive and negative control of proteasome degradation and miRNA biogenesis during neuronal development.
NeuN antibody staining is seen in most of the neurones in the mammalian CNS, with the exception of cerebellar Purkinje, olfactory bulb mitral, and retinal photoreceptor neurones. NeuN is not detected in tissues outside the nervous system or in glial cells or immature neural progenitor cells still undergoing the cell cycle. NeuN is also absent in diseased and dying neurones. The NeuN antibody is therefore a specific neuronal marker used to label post-mitotic neurones when studying the differentiation of stem cells and when quantifying neurones in normal and diseased states. Within neurones, NeuN antibody staining is localised in nuclei in areas of low chromatin density and perinuclear cytoplasm.