Antibody raised against Caenorhabditis elegans NDUF-11, the NDUFA11 homolog. NDUF-11 is part of mitochondrial complex I and the supercomplex; it is vital for complex I stability and normal respiratory function.
Detection and characterisation of nduf11a from (RNAi)-treated worms. A reduction of NDUF-11 protein in mitochondria after RNAi is detectable using the anti-nduf11a antibody. A total of 50 µg of isolated mitochondria is loaded per lane. Treatment with RNAi significant reduces detectable nduf11. Control: C. elegans mitochondria anti-nduf11a antibody detects Nduf11a. nduf-11: C. elegans mitochondria anti-nduf11a antibody detects a significant reduction in Nduf11a after RNAi treatment. ATP5 is used as a loading control. Primary: Anti-Nduf11a antibody (1: 500; 5% milk in TBS-T). Secondary: Anti-rabbit conjugated to HRP.
|Antigen Peptide||KLH conjugated synthetic peptide crb1200537e|
|Aliases||CELE_B0491.5, B14.7, CI-B14.7, NADH:ubiquinone oxidoreductase subunit A11, MC1DN14|
|Target Protein Species||C.Elegans|
|Concentration||1 mg/ml Glycine eluate|
|Target||B0491.5 C.Elegans (NDUFA11 homolog)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Disease Area||Encephalocardiomyopathy, Fatal infantile lactic acidemia, Research Area: Bioenergetics; electron transfer|
|Specificity||Subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Knapp-Wilson et al., (2021). Maintenance of complex I and its supercomplexes by NDUF-11 is essential for mitochondrial structure, function and health. J. Cell Sci., 134(13): jcs258399. PMID: 34106255.
Mitochondria contain 4 multimeric electron transfer complexes (CI-CIV) that allow a stepwise transfer of electrons leading to energy production. The electron transfer complexes can form supercomplexes with a core of monomeric CI and dimeric CIII. The purpose of the supercomplex is believed to be direct electron transfer in a discrete unit with additional structural roles in the mitochondria. NDUFA11 is a conserved integral membrane protein that sits at the interface of CI and CIII. Lack of functional NDUFA11 leads to infant death, and encephalocardiomyopathy.
Understanding NDUFA11 roles could be key to understanding mitochondrial diseases. This antibody is for the NDUFA11 homolog (NDUF-11) in the genetically tractable model Caenorhabditis elegans and has already provided insights. The C. elegans NDUF-11 subunit is integral for CI stability and the supercomplex. Loss of complex stability altered mitochondrial function, morphology and ultimately lead to premature death. Further work with this antibody will help understand NDUF-11 functions and possible roles in mitochondrial disorders.