Anti-MRGPR-X1 antibody
Description
Application Data
Description
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Recognises MRGPR-X1 in rat and mouse, MRGPR-X1 is a receptor involved in inducing acute pain
Application Data
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Western blot analysis of rat brain preparation.
Lane 1: Rat brain preparation (20µg).
Secondary: Goat anti-rabbit IgG conjugated to HRP 1:5000.
| Catalogue number | crb2005053 |
|---|---|
| Antibody | Anti-MRGPR-X1 antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200340e |
| Protein ID | UniProtKB - Q8R4G1 |
| Aliases | MAS Related GPR Family Member X1, Sensory Neuron-Specific G-Protein Coupled Receptor 3/4, MAS-Related GPR, Member X1, MRGX1, Mas-Related G-Protein Coupled Receptor Member X1, G Protein-Coupled Receptor MRGX1 |
| Cross-Reactivity | Rat, Mouse |
| Target Protein Species | Rat, Mouse |
| Host Species | Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 1mg/ml |
| Validation | 1:1000 (WB), 1:1000 (ELISA) |
| Target | MRGPR-X1 |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | MAS Related GPR Family |
| Disease Area | Inflammatory, Neurological, Cancer |
| Specificity | Protein |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Solinski, H., Petermann, F., Rothe, K., Boekhoff, I., Gudermann, T. and Breit, A. (2013). Human Mas-Related G Protein-Coupled Receptors-X1 Induce Chemokine Receptor 2 Expression in Rat Dorsal Root Ganglia Neurons and Release of Chemokine Ligand 2 from the Human LAD-2 Mast Cell Line. PLoS ONE, 8(3), e58756. PMID: 23505557
Solinski, H., Zierler, S., Gudermann, T. and Breit, A. (2012). Human Sensory Neuron-specific Mas-related G Protein-coupled Receptors-X1 Sensitize and Directly Activate Transient Receptor Potential Cation Channel V1 via Distinct Signaling Pathways. J Biol Chem, 287(49), 40956-40971. PMID: 23074220 |
Primate-specific Mas-related G protein-coupled receptor-X1 (MRGPR-X1) is highly enriched in dorsal root ganglia (DRG) neurones as well as in connective tissue mast cells (CTMC) and the leukaemia-derived human mast cell line (LAD)-2. MRGPR-X1 activation induces acute pain and therefore represents a promising target for future pain therapy.
MRGPR-X1 is activated by bovine adrenal medulla peptide-8-22 (BAM 8–22) which is cleaved from pro-enkephalin by pro-hormone convertases. Unlike most if not all Gq-coupled receptors MRGPR-X1 does not undergo agonist-promoted endocytosis.