Anti-JAK3 antibody

  • Description

  • Application Data

Description

An antibody raised against JAK3; a tyrosine kinase involved in cell growth, development, and differentiation. JAK3 is also vital for adaptive and innate immunity signalling cascades and has a critical role in haematopoiesis during T-cell development.

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Application Data

Catalogue number crb2005681
Antibody Anti-JAK3 antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200890
Protein ID UniProt KB - P52333
Aliases Tyrosine-protein kinase JAK3, Janus kinase 3 (JAK-3), Leukocyte janus kinase (L-JAK)
Cross-Reactivity Human
Host Species Rabbit
Antibody Type Polyclonal
Concentration 0.5 mg/ml Glycine (R1G), 1 mg/mL Glycine (R2G), 0.1 mg/mL TEA (R2T)
Target JAK3
Family Tyr Protein Kinase family (JAK subfamily)
Super Family Protein Kinase superfamily
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/thaw cycles.
References

Dullius et al., (2019). Are peptides a solution for the treatment of hyperactivated JAK3 pathways?. Inflammopharmacol, 27: 433. doi: 10.1007/s10787-019-00589-2.

Robinette et al., (2018). Jak3 deficiency blocks innate lymphoid cell development. Mucosal immunol.11(1): 50. doi: 10.1038/mi.2017.38.

Data Sheet Data Sheet Data Sheet Material Safety Data Sheet (MSDS)

Ligand binding to type I receptors with a common subunit gamma results in local cytoplasmic JAK3 being recruited to the cytoplasmic tails of the receptor. The tail becomes phosphorylated, creating STAT protein binding sites. The STAT complex then becomes phosphorylated to form a homo/heterodimer and translocates to the nucleus to bind to DNA and activate transcription. For example, interleukin IL2 binds to type I receptor ILR2 resulting in JAK1 with JAK3 recruitment to the cytoplasmic gamma subunit. This interaction leads to tyrosine phosphorylation of the receptor for Stat5A with Stat5B docking. These STATs are then phosphorylated and activated by JAK1 and JAK3 leading to their dimerisation and translocation for activation of specific gene transcription.

JAK3 also has vital roles in immune cell signalling pathways; IL2 binds IL2R on T cells and natural killer cells. It has been found that in these immune cells, JAK3 is critical to facilitate tyrosine phosphorylation of the cytoplasmic IL2R subunit. Throughout T cell differentiation, JAK3 is required for IL7-induced activation of IL7R to activate PI3k and STATs. Ablation of JAK3 completely halted T cell proliferation.  JAK3-deficient models have phenotypes like human severe combined immunodeficiency (SCID) due to the lack of developed lymphocytes. This was linked to the interaction between JAK3 and the T cell protein tyrosine phosphatases (TCPTP) key to cytokine signalling in haematopoietic cells.

Anti-JAK3 antibody

Cat No.Pack SizePriceQty.
20µg£50.00
20µg£50.00
20µg£50.00
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