Recognises C-terminus of HAND1, a twist family bHLH transcription factor essential for several developmental processes
Western blot analysis of HEK293T cell lysates with HAND-1 overexpression.
Lane 1: HEK293T cell lysates transiently transfected with TrueORF pCMV vector only (20µg)
Lane 2: HEK293T cell lysates transiently transfected with TrueORF pCMV-HAND-1 plasmid (20µg)
Secondary: Rabbit anti-sheep IgG 1:2000
* HAND-1 dimer and trimer at ~50kDa and ~75kDa
|Antigen Peptide||KLH conjugated synthetic peptide crb1200298f|
|Protein ID||UniProtKB - O96004|
|Aliases||Heart And Neural Crest Derivatives Expressed, Extraembryonic Tissues, Heart, Autonomic Nervous System And Neural Crest Derivatives-Expressed Protein, Class A Basic Helix-Loop-Helix Protein 27, BHLHa27, EHand, Heart- And Neural Crest Derivatives-Expressed|
|Cross-Reactivity||Human, Rat, Mouse|
|Target Protein Species||Human, Rat, Mouse|
|Validation||1:1000 (WB), 1:1000 (ELISA)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Barnes, R. and Firulli, A. (2009). A twist of insight – the role of Twist-family bHLH factors in development. Int J Dev Biol, 53(7), 909-924. PMID: 19378251
Firulli, B., Fuchs, R., Vincentz, J., Clouthier, D. and Firulli, A. (2014). Hand1 phosphoregulation within the distal arch neural crest is essential for craniofacial morphogenesis. Development, 141(15), 3050-3061. PMID: 25053435
Anti-Heart and Neural crest derivatives expressed transcript 1 (HAND-1) antibody corresponding to the C-terminus of the HAND-1 protein sequence. HAND1 is a basic helix-loop-helix (bHLH) transcription factor belonging to the twist family. Twist-family transcription factors play essential roles in a number of developmental programs and function by dimerising with other bHLH members and binding to cis- regulatory elements, called E-boxes. HAND1 can form homodimers as well as interact with other tissue restricted bHLH proteins, such as HAND2. Dimer partner choice infers differential regulation on twist family proteins and is regulated by phosphorylation. Protein Kinase A and C (PKA and PKC) can phosphorylate HAND1, while β56δ-containing protein phosphatase 2A (PP2A) can dephosphorylate HAND1.
HAND1 is required for proper cardiac morphogenesis and is essential for extra-embryonic and trophoblast cell differentiation. Hand1 is expressed in several foetal tissues and in adult heart tissue where it may have a role in preventing hypertrophy.