Recognises Grip71, a component of the γ-tubulin ring complex (γ-TuRC) from Drosophila in Western blots and immunohistochemistry
Immunofluorescence image of A. Grip71 Wild-type and B. Grip71 Mutant Drosophila larval brain cells stained with Anti-Grip71 antibody (crb2005268) in green. The DNA is stained in blue and the centrosomes are stained in magenta. Grip71 Mutant cell shows lack of centrosome staining.
Image provided courtesy of Dr. Paul T. Conduit
|Antigen Peptide||KLH conjugated synthetic peptide crb1200507e|
|Protein ID||UniProtKB - Q9VJ57_DROME|
|Target Protein Species||Drosophila|
|Validation||WB (1:1000), ELISA (1:1000), Immunoprecipitation|
|Target||Grip71-PA (single isoform gene)|
|Storage Stabilisers||The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
|Disease Area||Cytoskeleton, Microtubles, gamma-tubulin ring complex|
|Storage||The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
Tovey, C., Tubman, C., Hamrud, E., Zhu, Z., Dyas, A., Butterfield, A., Fyfe, A., Johnson, E. and Conduit, P. (2018). γ-TuRC Heterogeneity Revealed by Analysis of Mozart1. Current Biology, 28(14), pp.2314-2323.e6
Gunawardane, R., Martin, O. and Zheng, Y. (2003). Characterization of a New γTuRC Subunit with WD Repeats. Mol Biol Cell, 14(3), 1017-1026. PMID: 12631720
Romé, P. and Ohkura, H. (2018). A novel microtubule nucleation pathway for meiotic spindle assembly in oocytes. J Cell Biol, 217(10), 3431-3445. PMID: 30087124
Grip71 was first identified in Drosophila and named Drosophila gamma ring protein with WD repeats (Dgp71WD). It is a component of the γ-tubulin ring complex (γ-TuRC) and is a homologue of the mammalian NEDD1 (alternatively named GCP-WD). Despite the name, Grip71 lacks Grip domains and instead contains N-terminal WD40 repeats and a C-terminal domain with unknown structure required for Grip71’s association with the γ-TuRC. Grip71 recruits γ-TuRC to different microtubule organising centres (MTOCs).
Grip71 is recruited to the spindle equator and poles by Augmin. In mitotic cells, a loss of Grip71 or Augmin leads to a dramatic decrease in the spindle microtubule density.