Anti-Grb14 antibody
Description
Application Data
Description
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Antibody raised against Grb14; an adapter protein used to modulate coupling of cell surface receptor kinases with specific signalling pathways. Regulates PDPK1 membrane translocation in response to insulin, promoting transduction of insulin signals.
Application Data
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Material Safety Data Sheet (MSDS)Catalogue number crb2005655 Antibody Anti-Grb14 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200864e Protein ID Q14449 Grb14 Aliases Growth factor receptor-bound protein 14, GRB14 adapter protein Cross-Reactivity Human Target Protein Species Human Host Species Rabbit Antibody Type Polyclonal Concentration 2mg/ml Glycine (R1G) Target Grb14 Storage Stabilisers The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. Family GRB7/10/14 family Storage The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. References Cariou, B. (2004). Regulation and functional roles of Grb14. Frontiers in Bioscience, 9(1-3), p.1626.
Gondoin, A. et al. (2017). Identification of insulin-sensitizing molecules acting by disrupting the interaction between the Insulin Receptor and Grb14. Scientific Reports, 7(1).
Holt, L J. and Siddle, K. (2005). Grb10 and Grb14: enigmatic regulators of insulin action – and more? Biochemical Journal, 388(2), pp.393–406.
Growth factor receptor-bound protein 14 (Grb14) is the final described member of the Grb7 family of proteins, containing Grb7, Grb10 and Grb14. The Grb7 family all share a series of domains involved in protein-protein and protein-lipid interactions.
Under ligand induction, Grb14 binds to various receptor tyrosine kinases (RTKs), this interaction relies on the specific SH2 and PIR domains. These domains are likely a determinant of the specificity of Grb14 action.
Grb14-RTK interactions are established to be necessary for insulin (IR) receptors; by acting as an inhibitor of IR tyrosine kinase activity, Grb14-RTK complexes are capable of inhibiting insulin effects. Grb14 is a molecular adapter which can specifically bind to activated insulin receptors and inhibit its tyrosine kinase activity. Molecules capable of disrupting this binding have been shown to have potential as insulin sensitising agents.
Anti-Grb14 antibody
A high quality antibody against the T2A peptide
Andrew McKenzie and his team from the MRC Laboratory of Molecular Biology (LMB) enlisted the expertise of the team at Cambridge Research Biochemicals (CRB) to produce a highly specific antibody to the 2A peptide sequence. 2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus) and is used in cloning vectors. The team at MRC…