Recognises GLT1, the major glutamate transporter in the brain in rat, with human and mouse cross reactivity
Western blot analysis of rat brain preparation.
Lane 1: Rat brain preparation (20µg).
Secondary: Goat anti-rabbit IgG conjugated to HRP dilution 1:5000.
* GLT-1 dimer at ~124 kDa
|Antigen Peptide||KLH conjugated synthetic peptide crb1200000e|
|Protein ID||SwissProt - P60709|
|Aliases||GLT1, EAAT2 , Excitotoxic Amino Acid Transporter 2, Excitatory Amino Acid Transporter 2, Solute Carrier Family 1 Member 2, Sodium-Dependent Glutamate/Aspartate Transporter 2, Glutamate/Aspartate Transporter II|
|Target Protein Species||Mouse, Human|
|Validation||1:1000 (WB), 1:1000 (ELISA)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Disease Area||Neurodegenerative diseases|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Al Awabdh, S., Gupta-Agarwal, S., Sheehan, D., Muir, J., Norkett, R., Twelvetrees, A., Griffin, L. and Kittler, J. (2016). Neuronal activity mediated regulation of glutamate transporter GLT-1 surface diffusion in rat astrocytes in dissociated and slice cultures. Glia, 64(7), 1252-1264. PMID: 27189737
Meabon, J., Lee, A., Meeker, K., Bekris, L., Fujimura, R., Yu, C., Watson, G., Pow, D., Sweet, I. and Cook, D. (2011). Differential Expression of the Glutamate Transporter GLT-1 in Pancreas. J Histochem Cytochem, 60(2), 139-151. PMID: 22114258
Glutamate transporter 1 (GLT-1) is the major glutamate transporter in the brain, and is predominantly expressed in astrocytes, but at lower levels also in excitatory terminals. GLT-1 is best understood for its critical role in preventing neuronal excitotoxicity and brain seizures in the central nervous system (CNS). Maintaining the correct level of extracellular glutamate is crucial for neuronal transmission and network activity.
Glutamate uptake function allows GLTs/EAATs to terminate and shape excitatory synaptic transmission, and prevent neuronal excitotoxicity.Increasing evidence argues that GLT-1 also modulates, and is modulated by, metabolic processes that influence glucose homeostasis