Recognises the Glutamate-Aspartate Transporter (GLAST) in Western blots and immunohistochemistry with low background signal
Western blot analysis of rat brain preparation.
Lane 1: Rat brain preparation (10µg).
Secondary: Goat anti-rabbit IgG conjugated to HRP 1:2000.
* GLAST dimer at ~120 kDa
|Antigen Peptide||KLH conjugated synthetic peptide crb1200276e|
|Protein ID||UniProtKB - P24942|
|Aliases||Anti-EAAT1 Antibody, Anti-Excitatory Amino Acid Transporter 1 Antibody, Anti-Glutamate Transporter Antibody, Anti-SLC1A3 Antibody|
|Target Protein Species||Rat, Mouse|
|Target||Glutamate Transporter GLAST|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Family||sodium:dicarboxylate (SDF) symporter family.|
|Disease Area||Neurodegenerative diseases|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Martin, C., Houitte, D., Guillermier, M., Petit, F., Bonvento, G. and Gurden, H. (2012). Alteration of sensory-evoked metabolic and oscillatory activities in the olfactory bulb of GLAST-deficient mice. Front Neural Circuits, 6. PMID: 22291618
Perkins, E., Clarkson, Y., Suminaite, D., Lyndon, A., Tanaka, K., Rothstein, J., Skehel, P., Wyllie, D. and Jackson, M. (2018). Loss of cerebellar glutamate transporters EAAT4 and GLAST differentially affects the spontaneous firing pattern and survival of Purkinje cells. Hum Mol Genet, 27(15), 2614-2627. PMID: 29741614
Siddiqi, F., Chen, F., Aron, A., Fiondella, C., Patel, K. and LoTurco, J. (2012). Fate Mapping by PiggyBac Transposase Reveals That Neocortical GLAST+ Progenitors Generate More Astrocytes Than Nestin+ Progenitors in Rat Neocortex. Cereb Cortex, 24(2), 508-520. PMID: 23118195
GLAST/ EAAT-1 (GLutamate–ASpartate Transporter/ Excitatory Amino Acid transporter 1) (rodent/human nomenclature) is a sodium-dependent plasma membrane glutamate transporter expressed exclusively by astrocytes in the cerebellum and present at high densities near excitatory synapses. Glutamate transporters regulate glutamate receptors and limit glutamate accumulation to prevent neurotoxicity whilst ensuring accurate synaptic communication. GLAST is the major transporter expressed during development.
Loss of GLAST/EAAT-1 has been linked to the pathogenesis of several disorders affecting the motor system including several subtypes of spinocerebellar ataxia (SCA); SCA1, SCA5, SCA7, episodic ataxia type 6, spinal muscular atrophy and fragile X associated tremor/ataxia syndrome. Furthermore, disrupted GLAST/EAAT-1 has been associated with schizophrenia and cerebellar dysfunction and also is linked to the pathophysiology of Alzheimer’s disease, autism and other cognitive and neuropsychiatric disorders.