Anti-ERK1/2 antibody
Description
Application Data
Description
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Recognises ERK1/2 a MAPK family protein which controls cell proliferation
Application Data
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Western blot analysis of whole cell extract of Mouse embryonic Fibroblasts (MEFs)
| Catalogue number | crb2005025 |
|---|---|
| Antibody | Anti-ERK1/2 antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200306e |
| Protein ID | UniProtKB - P27361 |
| Aliases | Extracellular Signal-Related Kinase 1, ERK1, ERK-1, ERK2, ERK-2, Mitogen-Activated Protein Kinase 3, MAP Kinase 3, MAPK 3. |
| Cross-Reactivity | Human, Rat, Mouse |
| Target Protein Species | Human, Rat, Mouse |
| Host Species | Anti-Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 1mg/ml |
| Validation | 1:1000 (WB), 1:1000 (ELISA) |
| Target | ERK1/2 |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | Mitogen-activated protein (MAP) kinases family |
| Specificity | ERK1 and ERK2 |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Cook, S., Stuart, K., Gilley, R. and Sale, M. (2017). Control of cell death and mitochondrial fission by ERK1/2 MAP kinase signalling. FEBS J, 284(24), 4177-4195. PMID: 28548464
Zou, J., Lei, T., Guo, P., Yu, J., Xu, Q., Luo, Y., Ke, R. and Huang, D. (2018). Mechanisms shaping the role of ERK1/2 in cellular senescence (Review). Mol Med Rep, 19(2), 759–770. PMID: 30535440 |
Extracellular signal-regulated kinase 1/2 (ERK1/2) is a mitogen-activated protein kinase (MAPK) family protein and part of the Ras-Raf‐MEK‐ERK signalling pathway which plays a key role in controlling cell proliferation, differentiation and cell survival. ERK1/2 acts downstream of activated growth factor receptors, RAF protein kinases and mitogen-activated protein kinase kinases 1 and 2 (MEK1/2). MEK1 and MEK2 activate ERK1/2 by phosphorylation and once activated ERK1/2 enters the nucleus and phosphorylates transcription factors to induce changes in gene expression. In addition to this active ERK1/2 also translocates to other organelles including the endoplasmic reticulum, endosomes, golgi and mitochondria where it influences cell physiology. Overall ERK1/2 phosphorylates more than 200 different substrates including other protein kinases, transcription factors, RNA‐binding proteins, regulators of mRNA translation and regulators of cell death. ERK1/2 pathway is strongly implicated in cancer where its hyperactivation underpins the growth and maintenance of many tumour types.