An antibody raised against E3 ubiquitin-protein ligase (DZIP3); E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation.
Catalogue number crb2005752 Antibody Anti-DZIP3 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200961 Protein ID UniprotKB - Q86Y13 (Human) Aliases E3 ubiquitin-protein ligase DZIP3, DAZ-interacting protein 3, RING-type E3 ubiquitin transferase DZIP3, RNA-binding ubiquitin ligase of 138 kDa (hRUL138) Cross-Reactivity Human Host Species Rabbit Antibody Type Polyclonal Concentration 1 mg/ml Glycine Target E3 ubiquitin-protein ligase DZIP3 Storage Stabilisers The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. Storage The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. References
Kolapalli et al., (2021). RNA-Binding RING E3-Ligase DZIP3/hRUL138 Stabilizes Cyclin D1 to Drive Cell-Cycle and Cancer ProgressionDZIP3 Is a Novel Driver of Cell-Cycle and Cancer Progression. Cancer research, 81(2): 315. doi: 10.1158/0008-5472.CAN-20-1871.
Qiu et al., (2023). Proteomic analysis of DZIP3 interactome and its role in proliferation and metastasis in gastric cancer cells. ECR, 425(1): 113525. doi: 10.1016/j.yexcr.2023.113525.
E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme as a thioester and then directly transfer the ubiquitin to targeted substrates. DZIP3 is located in the cytoplasm and can specifically bind RNA. DZIP3 enables several functions, including phosphatase binding activity; polyubiquitin modification-dependent protein binding activity; and ubiquitin-protein transferase activity. In addition, DZIP3 is involved in protein polyubiquitination.
The condition of azoospermia is associated with the deletion of DZIP3. High expression of DZIP3 is a biomarker for a poor outcome of several cancers, including gastric and breast cancer. DZIP3 is a crucial driver of cancer cell growth, migration, and invasion due to interacting with cyclin D1 during G1. In breast cancer, DZIP3 is a coactivator of oestrogen receptor α target gene expression and enhances their expression in breast cancer cells.