Dixin activates DVL2 leading to Wnt3a signalling activation. Dixin was found to be an inhibitory regulator of JNK activity via AXIN1 and DVL2.  A catalogue of evidence suggests dysregulation of DIXDC1 is involved in the progression and development of a wide variety of cancers including acute myeloid leukaemia (AML), gastric, colon, and retinoblastoma. The role between DIXDC1 regulation of the Wnt pathway cancer cells remains unclear.

Dixin also associates with gamma tubulin at the centrosome, phosphorylation of Dixin has been shown to regulate its function in actin and microtubule cytoskeleton dynamics. Evidence suggests that Dixin is associated with chromosome segregation and cell cycle regulation.

Multiple Dixin isoforms have been identified. Mark1 was identified as key to phosphorylating the various isoforms to regulate dendrite and spine development. Autism spectrum disorders variations in DIXDC1 isoform 1 have been linked to impaired phosphorylation and neuronal morphology.