Anti-Cyclin D1 antibody
Recognises Cyclin D1, a key regulator of cell proliferation and tumourigenesis
Western blot analysis of whole cell extract of human prostate adenocarcinoma (PC-3) and human osteocarcinoma (U-2 OS) (12µg).
Lane 1: Human PC-3 protein (12µg).
Lane 2: Human U-2 OS protein (12µg).
Secondary: Goat anti-rabbit IgG conjugated to HRP 1:5000.
Western blot image provided courtesy of Prof. Neil Perkins Laboratory
|Antibody||Anti-Cyclin D1 antibody|
|Antigen Peptide||KLH conjugated synthetic peptide crb1200305e|
|Protein ID||UnitProtKB - P24385|
|Target Protein Species||Human|
|Validation||1:500 (WB), 1:500 (ELISA)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Disease Area||Cancer, cell cycle|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Pysz, M., Hao, F., Hizli, A., Lum, M., Swetzig, W., Black, A. and Black, J. (2014). Differential Regulation of Cyclin D1 Expression by Protein Kinase C α and ϵ Signaling in Intestinal Epithelial Cells. J Biol Chem, 289(32), 22268-22283. PMID: 24914206
Qie, S. and Diehl, J. (2016). Cyclin D1, cancer progression, and opportunities in cancer treatment. J Mol Med, 94(12), 1313-1326. PMID: 27695879
Cyclin D1 is a key regulator of cell proliferation and its expression and accumulation within cells is under tight control. Cyclin D1 is the regulatory subunit of cyclin-dependent kinases 4 and 6. These activated cyclin dependent kinases then phosphorylate the retinoblastoma protein (Rb) and drive G1 to S phase progression. Cyclin D1 promotes cell proliferation through interaction with transcription factors such as the oestrogen receptor and specificity protein 1 (Sp1). Cell proliferation is extremely sensitive to altered levels of cyclin D1, with even modest changes in its expression having noticeable effects on cell cycle progression.
Cyclin D1 is a proto-oncogene and its overexpression is one of the most frequent alterations seen in multiple cancer types.