Recognises the Drosophila cryptocephal protein, a bZIP transcription factor involved in insect moulting behaviour
Anti-crc antibody (crb2005253) (1:1000)
Western blot analysis of nuclear extract of Drosophila Schneider 2 cells (S2 cells) (50 ug) treated with 2mM DTT to induced by ER stress.
Lane 1: S2 cells
Lane 2: S2 cells treated with 2mM DTT for 1 hour
Lane 3: S2 cells treated with 2mM DTT for 6 hours
Secondary: IRDye® 800CW Goat anti-Rabbit IgG 1:2000
|Antigen Peptide||Recombinant protein|
|Protein ID||UniProtKB - Q9VID3_DROME|
|Cross-Reactivity||UniProtKB - Q9VID3_DROME|
|Target Protein Species||UniProtKB - Q9VID3_DROME|
|Validation||WB (1:1000), ELISA (1:1000)|
|Storage Stabilisers||The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
|Family||basic-leucine zipper transcription factors, CREB/ATF family of basic-leucine zipper transcription factors|
|Disease Area||Cell stress signalling, Integrated stress response, Unfolded protein response, Developmental signalling, Coffin-Lowry Syndrome, Leukoencephalopathy With Vanishing White Matter, Inflammatory bowel disease|
|Storage||The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
Malzer E., Dominicus C.S., Chambers J.E., Dickens J.A., Mookerjee S., Marciniak S.J. (2018). The integrated stress response regulates BMP signalling through effects on translation. BMC Biology 16:34. PMID: 29609607
Gauthier, S., Van Haaften, E., Cherbas, L., Cherbas, P. and Hewes, R. (2012). Cryptocephal, the Drosophila melanogaster ATF4, Is a Specific Coactivator for Ecdysone Receptor Isoform B2. PLoS Genet, 8(8), e1002883. PMID: 22912598
Hewes RS1, Schaefer AM, Taghert PH. (2000). The cryptocephal gene (ATF4) encodes multiple basic-leucine zipper proteins controlling molting and metamorphosis in Drosophila. Genetics. 155(4), 1711-1723. PMID: 10924469
Cryptocephal (CRC) is a basic-leucine zipper (bZIP) transcription factor in Drosophila and the homologue of vertebrate activating transcription factor 4 (ATF4), a member of the CREB/ATF family of transcription factors. CRC interacts with EcR-B2 to promote ecdysone-dependent expression of ecdysis triggering hormone (ETH), an essential regulator of insect moulting behaviour.
The crc gene is expressed throughout development and mutations in crc causes pleiotropic defects in ecdysone-regulated events during Drosophila moulting and metamorphosis. In third instar larvae, crc expression is high in targets of ecdysone signalling, such as the leg and wing imaginal discs and in the ring gland, where ecdysone is produced.