Anti-Cache Valley Virus (CVV) Nucleocapsid antibody
Description
Application Data
Description
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Recognises the viral capsid protein which encloses the nucleocapsids of Cache Valley virus and Batai virus
Application Data
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Western blot analysis of BHK-21 cells infected with a variety of orthobunyaviruses. The Anti- CVV Nucleocapsid antibody recognises nucleocapsid proteins in some members of the Bunyamwera serogroup.
Lane 1: Cache valley virus (Bunyamwera)
Lane 2: Kairi virus (Bunyamwera)
Lane 3: Bunyamwera virus (Bunyamwera)
Lane 4: Batai virus (Bunyamwera)
Lane 5: M’poko virus (Turlock)
Lane 6: Tacaiuma virus (Anopheles A)
Lane 7: Bwamba virus (Bwamba)
Lane 8: Guama virus (Guama)
Lane 9: Eretmapodites virus (Nyando)
Lane 10: Akabane virus (Simbu)
Lane 11: Schmallenberg virus (Simbu)
Secondary: Goat anti-rabbit IgG conjugated to HRP dilution 1:2000
Western blot image provided courtesy of Dr Alain Kohl’s Laboratory
| Catalogue number | crb2005018 |
|---|---|
| Antibody | Anti-Cache Valley Virus (CVV) Nucleocapsid antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200003e |
| Protein ID | UniProtKB - D6MXQ7 |
| Cross-Reactivity | Cache Valley Virus, Batai virus |
| Target Protein Species | Cache Valley Virus, Batai virus |
| Host Species | Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 1mg/ml |
| Validation | WB/ELISA |
| Target | Cache Valley Virus |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Specificity | Protein |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Citations | Dunlop et al., (2018) Development of reverse genetics systems and investigation of host response antagonism and reassortment potential for Cache Valley and Kairi viruses, two emerging orthobunyaviruses of the Americas. PLoS Negl Trop Dis. 12(10):e0006884. doi: 10.1371/journal.pntd.0006884. PMID: 30372452. |
| References | Wilson et al., (2017). A novel cause of chronic viral meningoencephalitis: Cache Valley virus. Ann Neurol, 82(1), 105-114. PMID: 28628941
Hoffmann et al., (2013). Ovine Fetal Immune Response to Cache Valley Virus Infection. J Virol, 87(10), 5586-5592. PMID: 23468505 |
Cache Valley virus (CVV) belongs to the Peribunyaviridae family of viruses (previously called Bunyaviridae) and is a mosquito born virus which is widespread throughout North and Central America. CVVs preferred mosquito vectors include several species of Aedes and it infects many species of domestic sheep and cattle. CVV infection in sheep causes spontaneous abortion, stillbirth, and congenital defects. The virus is neuroinvasive in humans and can cause central nervous system (CNS) diseases, such as encephalitis and meningitis and in rare cases has caused death. There appears to be a high infection rate (up to 18% of population) in virus-endemic areas. This antibody recognises the nucleocapsid protein in Cache Valley virus and Batai virus but not other members of the Bunyamwera serogroup such as Kairi virus and Bunyamwera virus.