Recognises B-raf kinase, a serine/threonine kinase and proto-oncogene involved in directing cell growth
Western blot analysis of whole cell extract of human Burkitt's lymphoma lymphoblasts (Daudi) (30 ug).
Lane 1: Daudi cells
Secondary: Goat anti-rabbit IgG conjugated to HRP (Vector) 1:2500
|Antigen Peptide||KLH conjugated synthetic peptide crb1200381e|
|Protein ID||UniProtKB - P15056|
|Aliases||Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, BRAF, RAFB1, P94, V-Raf Murine Sarcoma Viral Oncogene Homolog B|
|Cross-Reactivity||Human, rat, mouse, chicken|
|Target Protein Species||Human, rat, mouse, chicken|
|Validation||1:1000 (WB), 1:1000 (ELISA)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Family||TKL Ser/Thr protein kinase family. RAF subfamily.|
|Super Family||Protein kinase superfamily|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Eisenhardt, A., Sprenger, A., Röring, M., Herr, R., Weinberg, F., Köhler, M., Braun, S., Orth, J., Diedrich, B., Lanner, U., Tscherwinski, N., Tscherwinski, S., Schuster, S., Dumaz, N., Schmidt, E., Baumeister, R., Schlosser, A., Dengjel, J. and Brummer, T. (2016). Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles. Oncotarget, 7(18). PMID: 27034005
Li, Y., Takahashi, M. and Stork, P. (2013). Ras-mutant Cancer Cells Display B-Raf Binding to Ras That Activates Extracellular Signal-regulated Kinase and Is Inhibited by Protein Kinase A Phosphorylation. J Biol Chem, 288(38), 27646-27657. PMID: 23893412
B-Raf (BRAF) is a raf-family serine/threonine kinase and proto-oncogene involved in directing cell growth. The Raf kinases are subject to a complex activation process that integrates various protein-protein and protein–lipid interactions and positive as well as negative phosphorylation events. The Ras/Raf/mitogen-activated/extracellular-regulated kinase (MEK)/extracellular signal regulated kinase (ERK) pathway plays a pivotal role in controlling proliferation, survival and differentiation of metazoan cells.
B-Raf, the most potent kinase of the family, plays an important role in various developmental processes. B-Raf is also the most frequently mutated kinase in cancer, making it an important target for cancer treatment, especially in melanoma and hairy cell leukaemia. BRAF mutations are also found in the neuro-cardio-facio-cutaneous syndromes or RASopathies.